AI 中文总结
本研究以L-[1-13C]-缬氨酸为模型，利用SABRE技术实现部分水相介质中未修饰氨基酸的13C超极化，在1.1 T台式NMR上获超60倍信号增强，为其应用于甘氨酸提供初步证据。
AI 中文摘要
超极化技术可增强核磁共振（NMR）与磁共振成像（MRI）的灵敏度，实现对低浓度代谢物及动态过程的检测。可逆交换信号放大（SABRE）可从仲氢转移自旋极化，无需对底物进行永久性化学修饰。本研究以L-[1-13C]-缬氨酸为主要模型体系，展示了SABRE介导的未修饰氨基酸的13C超极化，并提供其适用于甘氨酸的初步证据；在1.1 T台式NMR谱仪上观测到超过60倍的信号增强，还探究了极化效果对仲氢鼓泡时间、溶剂组成及底物与催化剂比例的依赖关系。
英文摘要
Hyperpolarization techniques enhance the sensitivity of nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI), enabling detection of low concentration metabolites and dynamic processes. Signal Amplification by Reversible Exchange (SABRE) transfers spin polarization from parahydrogen without permanent chemical modification of the substrate. Here, we demonstrate SABRE-mediated 13C hyperpolarization of unmodified amino acids using L-[1- 13C]-valine as the primary model system and provide preliminary evidence of applicability to glycine. Signal enhancements exceeding 60-fold were observed on a 1.1 T benchtop NMR spectrometer. The dependence of polarization on parahydrogen bubbling time, solvent composition, and substrate-to-catalyst ratio was investigated.
