AI 中文总结
该研究提出并实验验证了一种集成端面微透镜的全多模光纤光镊系统，可实现多波长下细胞的稳定捕获与轴向动态操控，为光纤生物光子平台提供了新方案。
AI 中文摘要
光纤光镊在长距离操控、紧凑集成及生物环境微创操作方面具有独特优势。然而，多数光纤光镊依赖单模光纤（SMF），其受限于光模式多样性有限、操控灵活性降低。尽管多模光纤（MMF）支持更宽的传输模式谱，但其固有的低相干混合导模长期制约聚焦捕获构型的设计。为解决这些局限，我们提出并实验验证了一种完全基于MMF的光镊系统，该系统集成了在光纤端面制备的微透镜结构，可实现多波长下的稳定光捕获及被捕获细胞的动态操控。采用532 nm连续波激光器和800 nm飞秒激光器，我们证明两种光源均可通过高数值孔径（NA>0.7）的微透镜产生紧聚焦光斑，实现对细胞的稳定捕获和轴向动态操控。与传统基于SMF的光镊相比，该方法利用MMF的宽带和多模特性，支持波长灵活、动态可调的细胞捕获，为光纤实验室生物光子平台铺平了道路，该平台具有介入式操控、细胞分选、细胞荧光分析等潜在应用。
英文摘要
Optical fiber tweezers offer distinct advantages for long-distance manipulation, compact integration, and minimally invasive operation in biological environments. However, most optical fiber tweezers rely on single-mode fibers (SMFs), which are constrained by limited optical mode diversity and reduced control flexibility. Although multi-mode fibers (MMFs) support a wider spectrum of propagation modes, their inherent mixed guided modes with low coherence become a long-standing limitation for the design of focused trapping configurations. To address these limitations, we propose and experimentally validate a fully MMF-based optical tweezer system integrated with a micro-lens structure fabricated on the fiber facet, enabling stable optical trapping across multiple wavelengths and dynamic manipulation of trapped cells. Employing 532 nm continuous-wave and 800 nm femtosecond lasers, we demonstrate that both light sources can generate tightly focused optical spots through the micro-lens with a high numerical aperture (NA>0.7), achieving robust trapping and axial dynamic manipulation of cells. Compared with conventional SMF-based tweezers, this approach leverages the broadband and multi-mode properties of MMFs, allows for wavelength-flexible and dynamically adjustable trapping of cells, and paves the way for lab-on-fiber biophotonic platforms with potential applications such as interventional manipulation, cell sorting, and cellular fluorescence analysis.
