AI 中文总结
研究探索等离子体活化水（PAW）与酚类化合物联合对抗酵母的抗真菌活性，测定酚类化合物最低抑菌浓度，制备PAW-酚类溶液，发现PAW与肉桂酸和香草醛组合抗酵母活性最强，能有效抑制酵母生长，是控制真菌生长的有效可持续策略。
AI 中文摘要
有效的真菌控制对于防止食品、医疗和工业环境中的腐败、污染和感染至关重要。本研究探索了由瞬态火花放电产生的等离子体活化水（PAW）与天然酚类生物活性化合物联合对抗酿酒酵母和异常威克汉姆酵母的浮游生长、生物膜形成及表面粘附的抗真菌活性。测定酚类化合物最低抑菌浓度后，以1或2mg/mL浓度测试其对浮游生长的作用。生物膜在无菌载玻片上生长48小时，用吖啶橙或钙荧光白染色后用荧光显微镜检查。自来水经等离子体处理后立即制备PAW-酚类溶液。PAW与肉桂酸和香草醛的组合对浮游生长、生物膜形成和表面粘附表现出最强的抗酵母活性。暴露于PAW-酚类组合的生物膜明显破碎，残余细胞呈现结构损伤。当500μg/mL肉桂酸与PAW联合时观察到生物膜形成完全抑制。这些发现证明了将PAW与选定酚类化合物联合作为控制真菌生长的有效且可持续策略的潜力。
英文摘要
Effective fungal control is essential to prevent spoilage, contamination, and infections in food, healthcare, and industrial environments. This study explores the antifungal activity of plasma-activated water (PAW), generated by transient spark (TS) electrical discharge, combined with natural phenolic bioactive compounds against the planktonic growth, biofilm formation, and surface adhesion of the yeasts Saccharomyces cerevisiae and Wickerhamomyces anomalus. The phenolic compounds were tested at concentrations of 1 or 2 mg/mL for planktonic growth after determining their minimum inhibitory concentrations. Biofilms were grown on sterile microscope slides for 48 h, stained with acridine orange or calcofluor, and examined using fluorescence microscopy. PAW was used immediately after the plasma treatment of tap water to prepare PAW-phenolic solutions. The combinations of PAW with cinnamic acid and vanillin demonstrated the strongest antiyeast activity against planktonic growth, biofilm formation, and surface adhesion. Biofilms exposed to the PAW-phenolic combinations were notably fragmented, and the residual cells exhibited structural damage. Complete inhibition of biofilm formation was observed when 500 microg/mL cinnamic acid was combined with PAW. These findings demonstrate the potential of combining PAW with selected phenolic compounds as an effective and sustainable strategy for controlling fungal growth.
Comments12 pages, 8 figures
Journal refJ. Agric. Food Res., vol. 29, p. 103081, Jul. 2026
DOI：10.1016/j.jafr.2026.103081
