远程聚焦显微镜系统的三维视野
Three-dimensional field of view of remote focusing microscopy system
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中文总结 AI 辅助
研究远程聚焦显微镜系统三维视野，基于光线追踪模拟斯特列尔比评估视野，发现视野随样品平面与工作距离变化而变窄，速率取决于数值孔径，且通过实验交叉验证，还讨论了远程聚焦优缺点。
中文摘要 AI 辅助
远程聚焦（RF）显微镜以能在远离显微镜物镜工作距离的广泛平面上清晰成像而闻名。然而，其三维（3D）视野（FOV）的确切性质尚不清楚。在本信函中，我们报告了基于光线追踪对远程聚焦显微镜系统的视野研究。我们对两种远程聚焦系统配置的斯特列尔比进行了数值模拟，并用于评估3D视野。视野随着样品平面远离工作距离而逐渐变窄。为交叉验证模拟结果，我们在不同偏移平面上对视野进行了实验测量。这种变窄速率取决于数值孔径。我们还讨论了显微镜中远程聚焦的优缺点。
英文摘要
Remote focusing (RF) microscopy is well known for its ability to sharply image a wide range of planes away from the working distance of the microscope objective. However, the exact nature of the three-dimensional (3D) field of view (FOV) is not known. In this letter we report an optical ray tracing based FOV study of a remote focusing microscopy system. We numerically simulate the Strehl ratio of two configurations of the remote focusing systems, and use this for the evaluation of the 3D FOV. The FOV tapers down as the sample plane moves away from the working distance. To cross-verify our simulation results, we experimentally measured the FOV at various offset planes. This rate of taper depends on the numerical aperture. We also discuss the advantages and limitations of remote focusing in microscopy.
