AI 中文总结
研究旨在解决溶液中兆道尔顿聚集体异质性问题，核心方法是用纳米流体散射显微镜连续跟踪分子运动实现重复采样和时间平均，主要贡献是分辨率提高四倍，接近整体平均天然质谱性能。
AI 中文摘要
解决兆道尔顿聚集体的异质性需要在溶液中进行精确的单分子质量测量。质量光度法从单个分子表面着陆事件推断质量，而事件间测量的可变性限制了精度。纳米流体散射显微镜通过连续跟踪运动中的分子克服了这一限制，实现了对这些波动的重复采样和时间平均。用4.5 MDa DNA折纸进行基准测试表明，分辨率提高了四倍，接近整体平均的天然质谱性能。
英文摘要
Resolving heterogeneity in megadalton assemblies requires precise single-molecule mass measurements in solution. Mass photometry infers mass from individual molecular surface-landing events, and event-to-event measurement variability limits precision. Nanofluidic scattering microscopy overcomes this limitation by continuously tracking molecules in motion, enabling repeated sampling and temporal averaging of these fluctuations. Benchmarking with 4.5 MDa DNA origami demonstrates up to a fourfold improved resolution, approaching the performance of ensemble-averaged native mass spectrometry.
