通过减毒麻疹疫苗病毒诱导的Vero细胞机械软化
Mechanical Softening of Vero Cells Induced by an Attenuated Measles Vaccine Virus
AI总结：
研究利用AFM和共聚焦显微镜分析病毒感染后Vero细胞的机械响应，发现感染24小时内细胞弹性模量显著降低，表明细胞软化，同时观察到黏弹性行为的变化，揭示了细胞骨架重塑对感染过程中黏弹性特性的影响。
摘要：
生物物理改变的定量表征仍处于初级阶段。本研究利用原子力显微镜（AFM）结合共聚焦显微镜，研究了暴露于减毒麻疹疫苗病毒后的Vero细胞机械响应。AFM力距离测量在核周区域进行，以评估弹性及黏弹性质的变化。感染24小时内，感染乘数为0.5的细胞表现出Young模量中位数约35%的降低，表明显著的细胞软化。相应黏弹性行为的变化也有所观察，包括放松模量和黏性（效果与细胞骨架去聚化类似）。共聚焦显微镜进一步揭示了感染后细胞骨架的依赖性重组，表现为F-肌动蛋白分布的改变和丝状结构的变化。这些发现表明，细胞骨架重塑对感染过程中观察到的黏弹性特性变化有所贡献。本工作提出了一种简单且互补的方法，通过整合AFM与共聚焦成像来表征病毒与细胞的相互作用。
英文摘要：
Quantitative characterization of biophysical alterations caused by viral infection remains at an early stage. In this study, we examined the mechanical response of Vero cells after exposure to an attenuated Measles Vaccine Virus using atomic force microscopy (AFM) in combination with confocal microscopy. AFM force distance measurements were conducted in the perinuclear region to evaluate changes in elastic and viscoelastic properties. Within 24 hours post infection, cells infected at a multiplicity of infection of 0.5 exhibited an approximately thirty five percent decrease in median of the Young modulus relative to uninfected controls, indicating substantial cellular softening. Corresponding shifts in viscoelastic behavior were observed, including reductions in the relaxed modulus and in viscosities (effects comparable to those induced by cytochalasin D mediated actin depolymerization). Confocal microscopy further revealed a dependent reorganization of the cytoskeleton upon infection, marked by altered F actin distribution and changes in filament architecture. These findings suggest that actin remodeling contributes to the altered viscoelastic properties observed during infection. This work proposes a straightforward and complementary approach for characterizing virus cell interactions by integrating AFM with confocal imaging.
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