具有集成脂质密封飞升升体积室的有机电化学晶体管阵列用于同时电和光学检测膜蛋白活性
Organic Electrochemical Transistor Arrays with Integrated Lipid-Sealed Femtolitre Chambers for Simultaneous Electrical and Optical Detection of Membrane Protein Activity
AI总结：
本文提出一种方法，利用PEDOT:PSS有机电化学晶体管制作52个离子敏感的阵列，每个都集成有脂质密封的微孔，用于同时进行电和荧光显微镜研究膜蛋白活性。
摘要：
我们报告了一种方法，用于在玻璃盖玻片上制作52个离子敏感的PEDOT:PSS有机电化学晶体管阵列，每个都集成有氟聚合物微孔，该微孔通过脂质双层密封，可用于同时进行电和荧光显微镜研究膜蛋白活性。为了证明其能力，我们用含有20 μM Alexa-488染料和50 mM KCl的磷酸盐缓冲液填充微孔，用水-有机-水液体交换技术密封微孔，然后在密封的微孔上方填充不含染料的磷酸盐缓冲液，含100 mM KCl。我们插入α-溶血素，它嵌入脂质双层形成直径约为2.6 nm的七聚体孔道。该孔道允许K+离子扩散进入微孔和Alexa-488染料分子扩散出微孔，分别导致晶体管导电性和微孔荧光强度的相应下降。这两种信号在不同时间尺度上出现，与K+离子和Alexa-488分子的已知大小差异一致。我们的方法用于制作包含PEDOT:PSS OECTs的微孔阵列，这些OECTs分布于阵列中选定的微孔底部，具有可扩展性和多功能性，为使用更大阵列和与其他膜蛋白嵌入脂质双层密封微孔的研究开辟了道路。
英文摘要：
We report a scalable method for fabricating arrays of fifty-two ion-sensitive PEDOT:PSS organic electrochemical transistors (OECTs) on glass coverslips, each incorporating an integrated fluoropolymer microwell sealed with a lipid bilayer for simultaneous electrical and fluorescence microscopy studies of membrane proteins. The architecture addresses a key limitation of existing OECT arrays by providing every transistor with an independent fluid reservoir sealed by its own isolated segment of lipid bilayer, thereby confining membrane protein diffusion to a defined sensing region. Microwells containing a dye-labelled $50$~mM KCl solution were sealed with lipid bilayers and overlaid with a dye-free $100$~mM KCl solution. Following addition of $α$-hemolysin, formation of heptameric pores enabled K$^{+}$ ions to diffuse into the microwells and dye molecules to diffuse out, producing concurrent decreases in transistor conductance and fluorescence intensity. This integrated platform combines electrical and optical readout within independently addressable sensing sites and provides a versatile, scalable foundation for larger transistor arrays and the investigation of diverse membrane proteins reconstituted into lipid bilayers.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
