时间分辨多谱X射线计算机断层扫描用于冷冻保护剂在仿生材料中的扩散
Time-Resolved Multi-Spectral X-ray Computed Tomography of Cryoprotectant Diffusion Into Biomimetic Material
AI总结：
本文提出多谱X射线计算机断层扫描技术，用于非侵入性测量冷冻保护剂在仿生材料中的扩散分布，提高冷冻保存协议的准确性和诊断能力。
摘要：
通过玻璃化冷冻保存需要加载冷冻保护剂混合物。不足的加载可能导致冻结，阻碍成功复苏；过载则有毒。然而，现有的原位测量冷冻保护剂渗透仍大多未经验证，且无法确定个体冷冻保护剂浓度。我们引入多谱X射线计算机断层扫描（MSCT）以非侵入性量化冷冻保护剂扩散进入组织仿生材料的时空分布。开发的光子能级选择算法实现了对低对比度冷冻保护剂的灵敏度，无需对比剂或荧光边缘。该技术通过二甲基亚砜、甘油和水的混合物验证，将冷冻保护剂体积分数的分辨率控制在5%的准确度内。我们观察到冷冻保护剂在仿生水凝胶中的不均匀扩散现象，这一现象无法通过传统技术观测到。MSCT优于现有X射线CT方法，因为它不适用于多组分溶液，并且不隐含假设均匀扩散。这些进步使冷冻保护剂加载协议的系统发展成为可能，并提供诊断以在冷冻保存前评估玻璃化能力。
英文摘要：
Cryopreservation via vitrification requires loading cryoprotective cocktails. Insufficient loading may lead to freezing, precluding successful recovery; overloading is toxic. Yet, existing in situ measurements of cryoprotectant permeation remain largely unvalidated and do not resolve individual cryoprotectant concentrations. We introduce multi-spectral X-ray computed tomography (MSCT) to noninvasively quantify the spatiotemporal distribution of cryoprotectants diffusing into a tissue mimicking phantom. A developed photon-energy bin selection algorithm achieves sensitivity to low contrast cryoprotectants without contrast agents or fluorescence edges. The technique is validated with a dimethyl sulfoxide, glycerol, and water solution, resolving cryoprotectant volume fractions to within 5% accuracy. We observe heterogeneous diffusion of the cryoprotectants into the tissue mimicking hydrogel, a phenomenon not observable with conventional techniques. MSCT improves upon existing X ray CT methods because it is not underdetermined for multicomponent solutions and does not implicitly assume homogeneous diffusion. These advancements enable the systematic development of cryoprotectant loading protocols and provide diagnostics to assess vitrifiability before cryopreservation.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
