纳钻石赋能的扭力显微术揭示细胞-基质多维机械相互作用
Nanodiamond-Enabled Torsion Microscopy Uncovers Multidimensional Cell-Matrix Mechanical Interactions
AI总结：
纳钻石赋能的扭力显微术揭示细胞-基质相互作用中的扭力现象，提供高精度测量细胞机械模式，拓展生物力学研究应用。
摘要：
传统细胞力感知技术，如拉力力显微镜（TFM），主要局限于测量线性拉力，忽视且技术上无法捕捉细胞-基质相互作用中至关重要的纳米级扭力。在这里，我们介绍了一种纳钻石赋能的扭力显微术（DTM），它整合了氮空位（NV）中心作为方向标记与微柱阵列，以解耦并量化由细胞引起的纳米级旋转和翻译运动。该方法实现了高精度（~1.47度旋转精度和~3.13*10-15 Nm扭力灵敏度），能够重建细胞扭力力场和扭曲能量分布，这些此前被低估。我们的发现揭示了扭力在细胞-基质相互作用中的广泛存在，引入了
英文摘要：
Traditional cellular force-sensing techniques, such as traction force microscopy (TFM), are predominantly limited to measuring linear tractions, overlooking and technically unable to capture the nanoscale torsional forces that are critical in cell-matrix interactions. Here, we introduce a nanodiamond-enabled torsion microscopy (DTM) that integrates nitrogen-vacancy (NV) centers as orientation markers with micropillar arrays to decouple and quantify nanoscale rotational and translational motions induced by cells. This approach achieves high precision (~1.47 degree rotational accuracy and ~3.13*10-15 Nm torque sensitivity), enabling reconstruction of cellular torsional force fields and twisting energy distributions previously underestimated. Our findings reveal the widespread presence of torsional forces in cell-matrix interactions, introducing "cellular mechanical modes" where different adhesion patterns dictate the balance between traction- and torque- mediated mechanical energy transferred to the substrate. Notably, in immune cells like macrophages that generally exert low linear tractions, torque overwhelmingly dominates traction, highlighting a unique mechanical output for specific cellular functions. By uncovering these differential modes, DTM provides a versatile tool to advance biomechanical investigations, with potential applications in disease diagnostics and therapeutics.
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