用于评估储存红细胞的表面声波溶血测定法
Surface Acoustic Wave Hemolysis Assay for Evaluating Stored Red Blood Cells
AI总结：
本研究提出表面声波溶血测定法（SAW-HA）平台，以SAWHT为标志物评估储存红细胞，验证了其42天储存样本的检测有效性，发现BMI、代谢特征与溶血易感性相关，为输血现场即时红细胞质量评估提供了新方案。
摘要：
输血仍是现代医学的基石，每天挽救无数生命。然而，不同捐献者的血液质量差异极大——这一关键因素在临床实践中常被忽视。目前缺乏可在输血现场快速、台式、经济高效地评估储存红细胞（RBCs）的方法，且人们对储存红细胞质量的代谢特征与输血结局之间的关联仍存担忧。近期利用代谢组学方法评估储存红细胞的研究发现，捐献者的生物学特征（如遗传学、年龄、生活方式因素）是导致血液储存与输血异质性的根本原因。对捐献者内在因素的认识，为精准输血医学中评估储存质量、预测输血效果的方法提供了机遇。本文提出一种新平台——表面声波溶血测定法（SAW-HA），以表面声波溶血温度（SAWHT）作为红细胞质量标志物，用于储存红细胞的现场评估。我们证实，SAWHT是一种机制依赖、可重复的方法，可评估储存长达42天的人类红细胞。研究结果明确了6名捐献者中2名的红细胞具有独特的SAW溶血特征与代谢谱，其中高体重指数（BMI）和红细胞甘油三酯与溶血易感性升高相关。储存红细胞的代谢年龄——近期被认可的输血后效果预测指标——显示，2个低SAWHT单位的红细胞具有氧化还原调控紊乱、色氨酸代谢不足和高BMI的特征。综上，这些发现表明SAW-HA在输血医学中作为即时检测分析手段的潜力。
英文摘要：
Blood transfusion remains a cornerstone of modern medicine, saving countless lives daily. Yet the quality of transfused blood varies dramatically among donors-a critical factor often overlooked in clinical practice. Rapid, benchtop, and cost-effective methods for evaluating stored red blood cells (RBCs) at the site of transfusion are lacking, with concerns persisting about the association between metabolic signatures of stored RBC quality and transfusion outcomes. Recent studies utilizing metabolomics approaches to evaluate stored erythrocytes find that donor biology (e.g., genetics, age, lifestyle factors) underlies the heterogeneity associated with blood storage and transfusion. The appreciation of donor-intrinsic factors provides opportunities for precision transfusion medicine approaches for the evaluation of storage quality and prediction of transfusion efficacy. Here we propose a new platform, the Surface Acoustic Wave Hemolysis Assay (SAW-HA), for on-site evaluation of stored RBCs utilizing SAW Hemolysis Temperature (SAWHT) as a marker for RBC quality. We report SAWHT as a mechanism-dependent reproducible methodology for evaluating stored human RBCs up to 42 days. Our results define unique signatures for SAW hemolysis and metabolic profiles in RBCs from two of the six donors in which high body mass index (BMI) and RBC triglycerides associated with increased susceptibility to hemolysis. Metabolic age of the stored RBCs - a recently appreciated predictor of post-transfusion efficacy-reveal that RBCs from the two low SAWHT units were characterized by disrupted redox control, deficient tryptophan metabolism, and high BMI. Together, these findings indicate the potential of the SAW-HA as a point-of-care analysis for transfusion medicine.
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