整合网络药理学、代谢组学和肠道菌群分析以探讨金红片对慢性浅表性胃炎的作用
Integrating network pharmacology, metabolomics, and gut microbiota analysis to explore the effects of Jinhong tablets on chronic superficial gastritis
AI总结：
本研究整合网络药理学、代谢组学和肠道菌群分析，揭示了金红片通过调节脂质与能量代谢及甜菜碱等代谢物和特定肠道菌群，从而有效缓解慢性浅表性胃炎的作用机制。
摘要：
慢性浅表性胃炎（CSG）严重影响生活质量，并可能发展为更严重的胃部病理状态。中医药（TCM）能有效治疗CSG，以具有已知抗炎特性的金红片（JHT）为例，但其机制仍不清楚。本研究整合了网络药理学、非靶向代谢组学和肠道菌群分析，以探讨JHT如何缓解CSG。通过H&E染色建立并评估了大鼠CSG模型。我们鉴定了JHT的靶点谱并构建了多层生物分子网络。通过非靶向代谢组学测定了血浆中的差异代谢物，并通过16S rRNA测序评估了粪便和盲肠样本中的肠道菌群多样性与组成。JHT显著减轻了胃部炎症。网络药理学强调了代谢途径，特别是脂质和一氧化氮代谢，是JHT治疗作用的关键。代谢组学鉴定了关键差异代谢物，包括甜菜碱（增强肠道菌群）、磷脂和瓜氨酸（指示CSG严重程度）。通路富集支持了肠道菌群的参与。进一步的菌群分析表明，JHT增加了甜菜碱丰度，改善了短链脂肪酸的产生，并提升了Faecalibaculum和Bifidobacterium的丰度，从而缓解了胃部炎症。总之，JHT通过多种代谢过程（尤其是脂质和能量代谢）缓解CSG，并影响甜菜碱等代谢物以及Faecalibaculum和Bifidobacterium等肠道微生物。这些发现强调了JHT的治疗潜力，并加深了我们对中医药在CSG管理中作用的理解。
英文摘要：
Chronic superficial gastritis (CSG) severely affects quality of life and can progress to worse gastric pathologies. Traditional Chinese Medicine (TCM) effectively treats CSG, as exemplified by Jinhong Tablets (JHT) with known anti-inflammatory properties, though their mechanism remains unclear. This study integrated network pharmacology, untargeted metabolomics, and gut microbiota analyses to investigate how JHT alleviates CSG. A rat CSG model was established and evaluated via H&E staining. We identified JHT's target profiles and constructed a multi-layer biomolecular network. Differential metabolites in plasma were determined by untargeted metabolomics, and gut microbiota diversity/composition in fecal and cecal samples was assessed via 16S rRNA sequencing. JHT markedly reduced gastric inflammation. Network pharmacology highlighted metabolic pathways, particularly lipid and nitric oxide metabolism, as essential to JHT's therapeutic effect. Metabolomics identified key differential metabolites including betaine (enhancing gut microbiota), phospholipids, and citrulline (indicating severity of CSG). Pathway enrichment supported the gut microbiota's involvement. Further microbiota analysis showed that JHT increased betaine abundance, improved short-chain fatty acid production, and elevated Faecalibaculum and Bifidobacterium, thereby alleviating gastric inflammation. In conclusion, JHT alleviates CSG via diverse metabolic processes, especially lipid and energy metabolism, and influences metabolites like betaine alongside gut microbes such as Faecalibaculum and Bifidobacterium. These findings underscore JHT's therapeutic potential and deepen our understanding of TCM's role in CSG management.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
