新西兰 Aotearoa Waikite Valley 的微生物席宏基因组
Microbial Mat Metagenomes from Waikite Valley, Aotearoa New Zealand
AI总结：
本研究采集新西兰 Waikite Valley 温泉温度梯度上的微生物席样本，生成约 686 Gb 宏基因组数据并组装、分箱获得 MAGs，以支撑光养细菌基因组与嗜热群落生态比较研究。
摘要：
新元古代复杂多细胞生态系统兴起之前，是一个元古宙微生物生物圈；当时的生产力可能主要局限于由细菌构成的微生物席，这些细菌包括进行产氧光合作用的 Cyanobacteria（蓝细菌）、不产氧光养生物和异养生物。在现代环境中，类似的微生物席可见于碳酸盐潮坪和陆地温泉等受限环境。本文报道了来自新西兰 Aotearoa Waikite Valley 温泉中一个类似系统的宏基因组序列数据；那里富碳、弱碱性的地热水支撑着多样的光养微生物席。研究沿温泉流出通道的温度梯度断面，在从近源头约 62°C 至下游约 100 米处约 37°C 的 8 个点位进行了重复采样。利用 Illumina Novaseq 生成了约 686 Gb 的鸟枪法宏基因组序列数据。每个样本均使用 SPAdes 进行组装，随后通过 MetaBAT 进行宏基因组组装基因组（MAGs）分箱。这些数据可用于新型光养细菌的基因组分析，也可用于温度不同但其他条件相似的嗜热群落之间的生态比较。
英文摘要：
The rise of complex multicellular ecosystems Neoproterozoic time was preceded by a microbial Proterozoic biosphere, where productivity may have been largely restricted to microbial mats made up of bacteria including oxygenic photosynthetic Cyanobacteria, anoxygenic phototrophs, and heterotrophs. In modern environments, analogous microbial mats can be found in restricted environments such as carbonate tidal flats and terrestrial hot springs. Here, we report metagenomic sequence data from an analog in the hot springs of Waikite Valley, Aotearoa New Zealand, where carbon-rich, slightly-alkaline geothermal waters support diverse phototrophic microbial mats. The Waikite Valley hot spring in the Taupo Volcanic Zone of Aotearoa New Zealand was sampled in duplicate at 8 points along a temperature gradient transect of the outflow, from ~62 C (near the source) to ~37 C (~100 meters downstream). ~686 Gb of shotgun metagenomic sequence data was generated by Illumina Novaseq. Each sample was assembled using SPAdes, followed by binning of metagenome-assembled genomes (MAGs) by MetaBAT. These data are useful for the genomic analysis of novel phototrophic bacteria, as well as for ecological comparisons between thermophilic communities with varying temperatures but otherwise similar conditions.
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