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arXiv 2406.19989stat.MEq-bio.QM
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使用贝叶斯因子量化基因表达变化的双样本问题的闭式解

A Closed-Form Solution to the 2-Sample Problem for Quantifying Changes in Gene Expression using Bayes Factors

Franziska Hoerbst, Gurpinder Singh Sidhu, Melissa Tomkins, Richard J. Morris

AI总结:

本文针对RNA-Seq差异基因表达分析中的统计挑战,提出了一种基于共轭先验的闭式贝叶斯解,将复杂分析简化为计算贝叶斯因子的单一方程,用于对基因表达变化进行排序。

摘要:

测序技术已经彻底改变了分子生物学领域。我们现在有能力常规地获取组织样本中的完整RNA谱。这些丰富的数据允许对不同时间点的RNA水平进行比较分析,从而揭示发育过程的动态,并在不同环境响应下进行比较分析,为基因表达调控和应激反应提供见解。然而,鉴于来自生物学和技术来源的数据固有变异性,量化基因表达变化被证明是一个统计挑战。在此,我们提出了该问题的一个闭式贝叶斯解。我们的方法专为处理后的RNA-Seq数据的差异基因表达分析而定制。该框架将原本复杂的分析(通常涉及参数估计和多重统计检验)统一并简化为一个用于计算贝叶斯因子的简洁数学方程。利用共轭先验,我们可以解析地求解这些方程。对于每个基因,我们计算一个贝叶斯因子,该因子可用于根据给定RNA-Seq数据下基因表达变化的统计证据对基因进行排序。所提出的闭式解是在最小假设下推导的,并可应用于各种其他双样本问题。

英文摘要:

Sequencing technologies have revolutionised the field of molecular biology. We now have the ability to routinely capture the complete RNA profile in tissue samples. This wealth of data allows for comparative analyses of RNA levels at different times, shedding light on the dynamics of developmental processes, and under different environmental responses, providing insights into gene expression regulation and stress responses. However, given the inherent variability of the data stemming from biological and technological sources, quantifying changes in gene expression proves to be a statistical challenge. Here, we present a closed-form Bayesian solution to this problem. Our approach is tailored to the differential gene expression analysis of processed RNA-Seq data. The framework unifies and streamlines an otherwise complex analysis, typically involving parameter estimations and multiple statistical tests, into a concise mathematical equation for the calculation of Bayes factors. Using conjugate priors we can solve the equations analytically. For each gene, we calculate a Bayes factor, which can be used for ranking genes according to the statistical evidence for the gene's expression change given RNA-Seq data. The presented closed-form solution is derived under minimal assumptions and may be applied to a variety of other 2-sample problems.

机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。

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