用于三维成像的智能手机数字全息显微镜中的交互式缩放显示
Interactive zoom display in smartphone-based digital holographic microscope for 3D imaging
AI总结:
提出一种由3D打印光学组件、智能手机与Android应用构成的紧凑低成本DHM系统,可实时重建振幅和相位信息,并支持捏合手势交互缩放。
摘要:
数字全息术可在单次拍摄中同时获取显微样品的振幅和相位信息,因此有助于对透明物体(即可通过相位信息进行映射的相位物体)和运动物体的三维形状进行非接触、无创观察,在生物成像中具有应用价值。数字全息术与显微术的结合被称为数字全息显微术(DHM)。本研究提出了一种用于三维成像的基于智能手机的DHM系统,该系统结构紧凑、价格低廉,并能够实时观察物体;系统包含一个由3D打印机构建的光学系统,采用市售图像传感器和半导体激光器;此外,还使用一个基于Android的应用程序来重建该光学系统采集的全息图,从而勾勒出被观察物体的振幅和相位信息。同时,通过利用可扩展的衍射计算方法和触摸屏交互,我们通过捏合手势实现了缩放功能。研究结果表明,该DHM系统能够通过采集的全息图以近乎实时的方式成功获取被观察物体的振幅和相位信息。因此,本研究表明,可以构建一种低成本、紧凑的DHM系统,其中包括用于构建光学系统的3D打印机和用于重建全息图的智能手机应用程序。此外,这种基于智能手机的DHM系统能够实时采集、重建和显示全息图,展现了其相对于现有系统的优越性和新颖性。该系统还有望为生物学野外工作和偏远地区的病理诊断作出贡献。
英文摘要:
Digital holography has applications in bio-imaging because it can simultaneously obtain the amplitude and phase information of a microscopic sample in a single shot, thus facilitating non-contact, noninvasive observation of the 3D shape of transparent objects (phase objects, which can be mapped with the phase information,) and moving objects. The combination of digital holography and microscopy is called digital holographic microscopy (DHM). In this study, we propose a smartphone-based DHM system for 3D imaging that is compact, inexpensive, and capable of observing objects in real time; this system includes an optical system comprising a 3D printer using commercially available image sensors and semiconductor lasers; further, an Android-based application is used to reconstruct the holograms acquired by this optical system, thus outlining the amplitude and phase information of the observed object. Also, by utilizing scalable diffraction calculation methods and touchscreen interaction, we implemented zoom functionality through pinch-in gestures. The study results showed that the DHM system successfully obtained the amplitude and phase information of the observed object via the acquired holograms in an almost real time manner. Thus, this study showed that it is possible to construct a low cost and compact DHM system that includes a 3D printer to construct the optical system and a smartphone application to reconstruct the holograms. Furthermore, this smartphone-based DHM system's ability to capture, reconstruct, and display holograms in real time demonstrates its superiority and novelty over existing systems. This system is also expected to contribute to biology fieldwork and pathological diagnosis in remote areas.
机构、总结、中文摘要均由AI分析而来,可能存在错误,请以论文正文为准。