基于HET-s淀粉样纤维的仿生水凝胶用于原代海马神经元的长期培养
Biomimetic hydrogel based on HET-s amyloïd fibers for long-term culture of primary hippocampal neurons
AI总结：
本研究展示HET-s淀粉样纤维水凝胶作为多功能涂层，支持原代海马神经元体外培养超45天并具自发网络活动，且树突可深入渗透，为长期神经培养和体内植入提供理想材料。
摘要：
历史上，淀粉样纤维（AF）在研究中一直与退行性疾病相关联。然而，HET-s淀粉样纤维在形态和功能上与致病性淀粉样纤维（如α-突触核蛋白或aβ）几乎没有共同之处，并且近年来它们已成为生物涂层的候选材料。在这里，我们证明了HET-s淀粉样纤维水凝胶是一种用于原代海马神经元体外培养的极其多功能的涂层材料。首先，使用标准化ISO方案在体外证明了其无细胞毒性。然后，研究表明，在原代海马神经元在HET-s AF水凝胶上的体外培养可持续超过45天，并具有明显的自发网络活动特征，这是许多其他涂层尚未实现的壮举。最后，突出了细胞、树突和水凝胶之间的相互作用，表明树突可能能够深入渗透水凝胶，从而即使在微米厚的水凝胶内也能进行记录。最终，这些特性与使用标准生物化学技术进行的基团功能化相结合，使HET-s水凝胶成为神经元以及其他类型细胞长期生长的理想候选材料。这种多功能性和易用性绝对是前所未有的，尤其是对于蛋白质材料而言。由于其在与细胞外基质（ECM）接触时能从干膜转变为水凝胶的能力，它也可用于体内植入，解决了植入手术中水凝胶损伤的问题。
英文摘要：
Historically, amyloid fibers (AF) in research has always been linked to degenerative diseases. However, HET-s AF, by their morphology and function, have only little in common to pathogenic amyloid fibers such as α-synuclein or a\b{eta} and they have appeared as promising candidate for biocoating since few years. Here we have shown than HET-s amyloid fibers hydrogel is an extremely polyvalent coating material for the in vitro culture of primary hippocampal neurons. First, the non-cytotoxicity was demonstrated in vitro using standardized ISO protocols. Then, it is shown that in vitro culture of primary hippocampal neurons on HET-s AF hydrogels could last more than 45 days with clear signatures of spontaneous network activity, with which is a feat that not many other coatings have achieved yet. Finally, interactions between the cells, the dendrites and the hydrogels are highlighted, showing that dendrites might be able to penetrate the hydrogels in depth, therefore allowing recordings even within micrometer-thick hydrogels. In the end, those properties combined with group functionalization using standard biochemistry techniques, makes HET-s hydrogels ideal candidates to be used for the long-term growth of neurons as well as other types of cells. This versatility and easiness to use are definitely still unheard, especially for protein material. Due to its ability to transform from dry films to hydrogel when in contact with the extracellular matrix (ECM), it could also be used for in vivo implants, solving the issue of hydrogel damaging during the implant surgery.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
