基于短波红外光热显微术的毫米深度微米分辨率振动成像
Millimeter-deep micron-resolution vibrational imaging by shortwave infrared photothermal microscopy
AI总结：
本研究提出短波红外光热（SWIP）显微镜，通过泵浦碳-氢键泛音跃迁结合短波红外光热探测，实现毫米深度、亚微米分辨率的振动成像，光热信号幅度为光声信号的63倍，可分辨深层组织细胞内脂质与分层结构，填补相关成像技术空白。
摘要：
深层组织化学成像在生物和医学应用中发挥着至关重要的作用。本文提出了一种短波红外光热（SWIP）显微镜，可实现毫米深度的振动成像，具备亚微米横向分辨率和纳米颗粒检测灵敏度。通过泵浦碳-氢键的泛音跃迁，并用短波红外光探测随后产生的光热透镜，SWIP能够从位于高散射仿体中毫米深度的微粒获取化学对比度。通过对光学探测信号进行快速数字化处理，实验显示光热信号的幅度比光声信号大63倍，从而实现了对纳米级物体的高灵敏度检测。SWIP能够分辨完整肿瘤球体内的细胞内脂质，以及毫米厚的肝脏、皮肤、大脑和乳腺组织中的分层结构。综上，SWIP显微术填补了亚细胞分辨率、毫米级穿透深度的振动成像领域的空白，在生命科学和临床应用中展现出广阔的潜力。
英文摘要：
Deep-tissue chemical imaging plays a vital role in biological and medical applications. Here, we present a shortwave infrared photothermal (SWIP) microscope for millimeter-deep vibrational imaging with sub-micron lateral resolution and nanoparticle detection sensitivity. By pumping the overtone transition of carbon-hydrogen bonds and probing the subsequent photothermal lens with shortwave infrared light, SWIP can obtain chemical contrast from microparticles located millimeter-deep in a highly scattering phantom. By fast digitization on the optically probed signal, the amplitude of photothermal signal is shown to be 63 times larger than that of photoacoustic signal, thus enabling highly sensitive detection of nanoscale objects. SWIP can resolve the intracellular lipids across an intact tumor spheroid and the layered structure in millimeter-thick liver, skin, brain, and breast tissues. Together, SWIP microscopy fills a gap in vibrational imaging with sub-cellular resolution and millimeter-level penetration, which heralds broad potential for life science and clinical applications.
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