利用生物物理模型高通量设计培养组织模具
High-throughput design of cultured tissue moulds using a biophysical model
AI总结：
本文提出一种基于生物物理模型的高通量模具设计方法，通过模拟细胞-基质相互作用和组织尺度力，识别出能促进高度细胞排列的系绳模具设计，为培养组织应用提供概念验证。
摘要：
本文提出的技术能够识别出经过优化的系绳模具设计，用于培养具有高度排列细胞的培养组织。该技术基于一种针对极化细胞水凝胶的微观生物物理模型。目前，对于辅助培养组织生长（具有定制细胞组织）的模具和支架设计工具存在未满足的需求，这些组织可用于再生医学、药物筛选和培养肉等应用。我们对多种计算机生成的模具进行了高通量生物物理计算，使用收缩网络偶极子取向模型模拟了细胞-基质相互作用和组织尺度力。研究发现，具有中央加宽和以下系绳策略之一的细长模具可导致高度排列的细胞：（1）在短边凹陷产生的双侧突起内放置系绳，以引导排列；（2）在单个顶点内放置系绳，以缩小错排可用的空间。因此，基于最近开发的生物物理模型，已经展示了模具和系绳支架设计的概念验证。该方法适用于多种在组织中排列的细胞类型，并可扩展到3D支架。
英文摘要：
The technique presented here identifies tethered mould designs, optimised for growing cultured tissue with very highly-aligned cells. It is based on a microscopic biophysical model for polarised cellular hydrogels. There is an unmet need for tools to assist mould and scaffold designs for the growth of cultured tissues with bespoke cell organisations, that can be used in applications such as regenerative medicine, drug screening and cultured meat. High-throughput biophysical calculations were made for a wide variety of computer-generated moulds, with cell-matrix interactions and tissue-scale forces simulated using a contractile-network dipole-orientation model. Elongated moulds with central broadening and one of the following tethering strategies are found to lead to highly-aligned cells: (1) tethers placed within the bilateral protrusions resulting from an indentation on the short edge, to guide alignment (2) tethers placed within a single vertex to shrink the available space for misalignment. As such, proof-of-concept has been shown for mould and tethered scaffold design based on a recently developed biophysical model. The approach is applicable to a broad range of cell types that align in tissues and is extensible for 3D scaffolds.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
