采用一次性等离子体检测技术的蛋白质和病毒粒子多重生物传感
Multiplexed Biosensing of Proteins and Virions with Disposable Plasmonic Assays
AI总结：
针对现有诊断技术周转慢或检测靶点少的问题，本研究提出具备多重检测能力的低成本无标记一次性等离子体生物传感平台，验证了其生物传感性能与SARS-CoV-2检测能力，可用于近床旁多靶点筛查。
摘要：
我们日益增强的个性化医疗定制能力有望变革临床治疗模式。然而，为临床决策提供依据的多种生物标志物检测，需要快速、高效且经济的诊断手段。慢性疾病以及在更大规模人群中快速演化的病原体，也使得对更强诊断能力的需求不断升级。当前的化学诊断通常在集中式机构开展，仍依赖多步操作、分子标记和复杂分析，导致结果周转时间长达数小时甚至数天。基于侧向流装置的快速诊断试剂盒虽能快速出结果，但仅能检测少数几种病原体或标志物。本文中，我们提出将一次性等离子体技术作为具备多重检测能力的低成本无标记光学生物传感平台，省去了当前光学生物传感器通常必需的流体系统。我们展示了一套系统的生物传感能力，该系统利用手性光学测量，以及带有手里剑形纳米压痕的超薄膜产生的等离子体增强荧光效应。我们还在复杂溶液中实现了SARS-CoV-2的检测，这是迈向高通量、单步、可鉴别诊断多种呼吸道病毒及满足其他新兴诊断需求的诊断试剂盒的早期开发里程碑。基于我们命名为Disposable Plasmonics Assays（一次性等离子体检测）的这一平台的诊断技术，适用于近床旁场景下的多种病原体或生物标志物低成本筛查。
英文摘要：
Our growing ability to tailor healthcare to the needs of individuals has the potential to transform clinical treatment. However, the measurement of multiple biomarkers to inform clinical decisions requires rapid, effective, and affordable diagnostics. Chronic diseases and rapidly evolving pathogens in a larger population have also escalated the need for improved diagnostic capabilities. Current chemical diagnostics are often performed in centralised facilities and are still dependent on multiple steps, molecular labelling, and detailed analysis causing the result turnaround time to be over hours and days. Rapid diagnostic kits based on lateral flow devices can return results quickly but are only capable of detecting a handful of pathogens or markers. Herein we present the use of disposable plasmonics as a platform for low-cost label-free optical biosensing with multiplexing capabilities, eliminating the need for flow systems often required in current optical biosensors. We demonstrate the biosensing capabilities of a system that uses chiroptical measurements as well as plasmonic enhanced fluorescence from metafilms with shuriken shaped nanoindentations. We also perform the detection of SARS-CoV-2 in complex solutions as an early developmental milestone towards high-throughput, single-step, diagnostic kits for differential diagnosis of multiple respiratory viruses and any other emerging diagnostic needs. Diagnostics based on this platform which we term Disposable Plasmonics Assays, would be suitable for low-cost screening of multiple pathogens or biomarkers in a near point of care setting.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
