RNMT的综合性泛癌分析：一种潜在的预后和免疫学生物标志物
Integrative Pan-Cancer Analysis of RNMT: a Potential Prognostic and Immunological Biomarker
AI总结：
本研究通过多数据库综合分析发现RNMT在泛癌中普遍高表达，与多种癌症预后及免疫调节因子、TMB、MSI等密切相关，是潜在的预后和免疫学生物标志物。
摘要：
背景：RNA鸟嘌呤-7甲基转移酶（RNMT）是N7-甲基鸟苷的主要调节因子之一，RNMT的失调与肿瘤发生和免疫代谢相关。然而，RNMT在泛癌中的具体功能仍不清楚。方法：使用多个数据库分析不同癌症中的RNMT表达，包括Cancer Cell Line Encyclopedia（CCLE）、Genotype-Tissue Expression Project（GTEx）和The Cancer Genome Atlas（TCGA）。使用Cox回归分析和Kaplan-Meier分析来评估RNMT表达与预后的相关性。数据还用于研究RNMT表达与常见免疫调节因子、肿瘤突变负荷（TMB）、微卫星不稳定性（MSI）、错配修复（MMR）和DNA甲基转移酶（DNMT）之间的关系。此外，使用cBioPortal网站评估RNMT改变的特征。使用TISDB数据库获取不同亚型的表达。使用Tumor Immune Estimation Resource（TIMER）数据库分析RNMT与肿瘤免疫浸润之间的关联。使用基因集富集分析（GSEA）识别相关通路。结果：RNMT在各种癌症中普遍高表达，生存分析显示其表达与多种癌症类型的临床预后高度相关。值得注意的是，RNMT参与免疫调节并在肿瘤微环境中发挥关键作用。在结肠腺癌、肾透明细胞癌和肝细胞癌中发现RNMT表达与六种免疫细胞类型表达呈正相关。此外，RNMT表达在大多数癌症类型中与免疫调节因子高度相关，并与TMB、MSI、MMR和DNMT相关。最后，GSEA表明RNMT可能与肿瘤免疫相关。
英文摘要：
Background: RNA guanine-7 methyltransferase (RNMT) is one of the main regulators of N7-methylguanosine, and the deregulation of RNMT correlated with tumor development and immune metabolism. However, the specific function of RNMT in pan-cancer remains unclear. Methods: RNMT expression in different cancers was analyzed using multiple databases, including Cancer Cell Line Encyclopedia (CCLE), Genotype-Tissue Expression Project (GTEx), and The Cancer Genome Atlas (TCGA). Cox regression analysis and Kaplan-Meier analysis were used to estimate the correlation of RNMT expression to prognosis. The data was also used to research the relationship between RNMT expression and common immunoregulators, tumor mutation burden (TMB), microsatellite instability (MSI), mismatch repair (MMR), and DNA methyltransferase (DNMT). Additionally, the cBioPortal website was used to evaluate the characteristics of RNMT alteration. The TISDB database was used to obtain the expression of different subtypes. The Tumor Immune Estimation Resource (TIMER) database was used to analyze the association between RNMT and tumor immune infiltration. Gene set enrichment analysis (GSEA) was used to identify the relevant pathways. Results: RNMT was ubiquitously highly expressed across cancers and survival analysis revealed that its expression was highly associated with the clinical prognosis of various cancer types. Remarkably, RNMT participates in immune regulation and plays a crucial part in the tumor microenvironment. A positive association was found between RNMT expression and six immune cell types expression in colon adenocarcinoma, kidney renal clear cell carcinoma, and liver hepatocellular carcinoma. Moreover, RNMT expression was highly associated with immunoregulators in most cancer types, and correlated to TMB, MSI, MMR, and DNMT. Finally, GSEA indicated that RNMT may correlate with tumor immunity.
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