形态学鉴定的小鼠上丘神经元的在体全细胞记录
In vivo whole-cell recording from morphologically identified mouse superior colliculus neurons
摘要：
在体内全细胞记录结合生物胞素标记后的形态学表征是一个强大的技术，研究阈下突触处理的细胞类型确定的神经元群体。在这里，我们提供了一个循序渐进的程序进行全细胞记录在上丘的麻醉小鼠，在啮齿动物大脑中的主要视觉处理区域。描述了两种类型的视觉刺激方法。虽然我们专注于上丘神经元，但该协议适用于其他大脑区域。
英文摘要：
In vivo whole-cell recording when combined with morphological characterization after biocytin labeling is a powerful technique to study subthreshold synaptic processing in cell-type-identified neuronal populations. Here, we provide a step-by-step procedure for performing whole-cell recordings in the superior colliculus of urethane-anesthetized mice, a major visual processing region in the rodent brain. Two types of visual stimulation methods are described. While we focus on superior colliculus neurons, this protocol is applicable to other brain areas.
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