塔拉太平洋考察珊瑚礁生态系统特征的综合组学框架
Integrative omics framework for characterization of coral reef ecosystems from the Tara Pacific expedition
摘要：
珊瑚礁科学是一个快速发展的领域，因为需要更好地了解珊瑚的健康和恢复力，以制定战略来减缓环境压力造成的珊瑚礁损失。珊瑚复原力的关键是在一个复杂的holobiont内建立的共生相互作用，即由宿主珊瑚有机体、内共生甲藻、细菌、古细菌、真菌和病毒组成的多体组合。塔拉太平洋是一个雄心勃勃的项目，建立在以前的塔拉海洋探险的经验，并利用国家的最先进的测序技术和分析，以解剖珊瑚holobiont筛选在大多数群岛遍布整个太平洋的生物多样性和生物复杂性。在这里，我们详细介绍了Tara Pacific多组学数据生成的工作流程，从样品处理到核苷酸序列数据生成和沉积。这一独特的多维框架还包括大量并行收集的伴随元数据，这些元数据提供了对珊瑚礁生物多样性（包括微生物多样性）的新评估，并塑造了未来对珊瑚礁动态及其在人类世中命运的调查。
英文摘要：
Coral reef science is a fast-growing field propelled by the need to better understand coral health and resilience to devise strategies to slow reef loss resulting from environmental stresses. Key to coral resilience are the symbiotic interactions established within a complex holobiont, i.e. the multipartite assemblages comprising the host coral organism, endosymbiotic dinoflagellates, bacteria, archaea, fungi, and viruses. Tara Pacific is an ambitious project built upon the experience of previous Tara Oceans expeditions, and leveraging state-of-the-art sequencing technologies and analyses to dissect the biodiversity and biocomplexity of the coral holobiont screened across most archipelagos spread throughout the entire Pacific Ocean. Here we detail the Tara Pacific workflow for multi-omics data generation, from sample handling to nucleotide sequence data generation and deposition. This unique multidimensional framework also includes a large amount of concomitant metadata collected side-by-side that provide new assessments of coral reef biodiversity including micro-biodiversity and shape future investigations of coral reef dynamics and their fate in the Anthropocene.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
