绿色荧光蛋白荧光各向异性的压力和温度依赖性
Pressure and temperature dependence of fluorescence anisotropy of Green Fluorescent Protein
摘要：
研究了高静压和温度对绿色荧光蛋白（GFP）稳态荧光各向异性的影响。我们发现，荧光各向异性的GFP在恒定的温度下，随着压力的增加而减少。在大气压下，各向异性随温度的升高而减小，但当压力大于20 MPa时，各向异性随温度的升高而呈现最大值。各向异性最大值对应的温度随压力的增加而增加。考虑到GFP的旋转相关时间的变化与压力-温度依赖的粘度的溶剂，我们认为，粘度随压力的增加是不是一个主要的贡献因素，各向异性随压力的下降。我们的研究结果表明，荧光各向异性随压力的下降可能是由于生色团周围的氢键环境的变化。
英文摘要：
We have studied the effect of high hydrostatic pressure and temperature on the steady state fluorescence anisotropy of Green Fluorescent Protein (GFP). We find that the fluorescence anisotropy of GFP at a constant temperature decreases with increasing pressure. At atmospheric pressure, anisotropy decreases with increasing temperature but exhibits a maximum with temperature for pressure larger than 20 MPa. The temperature corresponding to the maximum of anisotropy increases with increasing pressure. By taking into account of the rotational correlation time changes of GFP with the pressure-temperature dependent viscosity of the solvent, we argue that viscosity increase with pressure is not a major contributing factor to the decrease in anisotropy with pressure. Our results suggest that the decrease of fluorescence anisotropy with pressure may result from changes in H-bonding environment around the chromophore.
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