小鼠胃肠道高时空分辨率NIR-IIb成像
High Spatial and Temporal Resolution NIR-IIb Gastrointestinal Imaging in Mice
摘要：
传统的生物医学成像方式，包括内窥镜检查、X射线和磁共振，是侵入性的，并且不能为胃肠道（GI）的常规成像提供足够的空间和时间分辨率以指导GI疾病的预后和治疗。在这里，我们报告了一种非侵入性的胃肠道光学成像方法。它是基于一种新型的镧系元素掺杂的荧光粉，具有980 nm的近红外（NIR）激发和1530 nm附近的第二近红外窗口（NIR-IIb）（1500~1700 nm）荧光发射。通过合理设计和控制合成高亮度的纳米晶，使其绝对量子产率（QY）达到48.6%。进一步受益于通过NIR-IIb窗口的最小化散射，与用于胃肠道成像的其他NIR-IIa（1000~1500 nm）对比剂相比，我们将空间分辨率提高了3倍。该方法还导致了每秒8帧的高时间分辨率，因此可以捕获1分钟内发生的小鼠肠道肿胀的瞬间。此外，与光片成像系统，我们展示了一个三维（3D）成像的立体结构的胃肠道。此外，我们成功地将这些进展转化为在小鼠结肠炎的临床前模型中诊断炎症性肠病（IBD）。
英文摘要：
Conventional biomedical imaging modalities, including endoscopy, X-rays, and magnetic resonance, are invasive and cannot provide sufficient spatial and temporal resolutions for regular imaging of gastrointestinal (GI) tract to guide prognosis and therapy of GI diseases. Here we report a non-invasive method for optical imaging of GI tract. It is based on a new type of lanthanide-doped nanocrystal with near-infrared (NIR) excitation at 980 nm and second NIR window (NIR-IIb) (1500~1700 nm) fluorescence emission at around 1530 nm. The rational design and controlled synthesis of nanocrystals with high brightness have led to an absolute quantum yield (QY) up to 48.6%. Further benefitting from the minimized scattering through the NIR-IIb window, we enhanced the spatial resolution by 3 times compared with the other NIR-IIa (1000~1500 nm) contract agents for GI tract imaging. The approach also led to a high temporal resolution of 8 frames per second, so that the moment of mice intestinal peristalsis happened in one minute can be captured. Furthermore, with a light-sheet imaging system, we demonstrated a three-dimensional (3D) imaging of the stereoscopic structure of the GI tract. Moreover, we successfully translate these advances to diagnose inflammatory bowel disease (IBD) in a pre-clinical model of mice colitis.
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