为什么一个扩散的单分子可以在几分钟内被一个大的捕获生物电子接口检测到
Why a diffusing single-molecule can be detected in few minutes by a large capturing bioelectronic interface
摘要：
在毫微微摩尔溶液中的纳米界面处的单分子检测可能需要数周时间，因为待检测的扩散分子与转导纳米装置之间的相遇率小得可以忽略不计。另一方面，一些实验证明，基于场效应晶体管（FET）的宏观无标记传感器，接合微米或毫米检测接口能够在几分钟内测定大体积中的单个分子。目前的工作证明了为什么在100 μ l体积中扩散的几个分子中的至少一个分子具有非常高的概率击中大的捕获和检测电子界面。为此，这里提供了用电解质门控FET测量的感测数据以及基于布朗扩散的建模，所述FET的栅极用1012捕获抗免疫球蛋白G功能化。EG-FET测定浓度低至几十μ M的溶液，体积范围为25 μ l至1 ml，其中将功能化的门温育30 s至20 min。
英文摘要：
Single-molecule detection at a nanometric interface in a femtomolar solution, can take weeks as the encounter rate between the diffusing molecule to be detected and the transducing nano-device is negligibly small. On the other hand, several experiments prove that macroscopic label-free sensors based on field-effect-transistors (FET), engaging micrometric or millimetric detecting interfaces are capable to assay a single-molecule in a large volume within few minutes. The present work demonstrates why at least a single molecule out of a few diffusing in a 100 ul volume has a very high probability to hit a large capturing and detecting electronic interface. To this end, sensing data, measured with an electrolyte-gated FET whose gate is functionalized with 1012 capturing anti-immunoglobulin G, are here provided along with a Brownian diffusion-based modelling. The EG-FET assays solutions down to some tens of zM in concentrations with volumes ranging from 25 ul to 1 ml in which the functionalized gates are incubated for times ranging from 30 s to 20 min.
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