蛋白质组学标准倡议ProForma 2.0统一蛋白质型和肽型的编码
Proteomics Standards Initiatives ProForma 2.0 Unifying the encoding of Proteoforms and Peptidoforms
摘要：
需要以标准方式表示蛋白质或肽一级序列的所有可能的变化，包括肽和蛋白质的人工修饰和翻译后修饰。基于这一总体目标，人类蛋白质组组织（HUPO）蛋白质组学标准倡议（PSI）开发了一种称为ProForma 2.0的符号，它是由自顶向下蛋白质组学联盟（CTDP）开发的原始ProForma符号的实质性扩展。ProForma 2.0旨在统一Proteoforms和Peptidoforms的表示。因此，这种符号支持自下而上和中间/自上而下蛋白质组学方法所需的用例，并允许使用人类和机器可读的字符串编码高度修饰的蛋白质和肽。ProForma 2.0涵盖了编码蛋白质修饰名称和登录、交联试剂（包括二硫化物、聚糖）、使用质量位移和/或通过化学式编码的修饰、不稳定和C或N末端修饰、修饰位置的模糊性和原子同位素的表示等用例。符号约定基于公共控制的词汇表和本体。有关符号和现有实现的详细信息，请访问www.example.com和相应的GitHub存储库（https：//github.com/HUPO-PSI/proforma）。
英文摘要：
There is the need to represent in a standard manner all the possible variations of a protein or peptide primary sequence, including both artefactual and post-translational modifications of peptides and proteins. With that overall aim, here, the Human Proteome Organization (HUPO) Proteomics Standards Initiative (PSI) has developed a notation, called ProForma 2.0, which is a substantial extension of the original ProForma notation, developed by the Consortium for Top-Down Proteomics (CTDP). ProForma 2.0 aims to unify the representation of proteoforms and peptidoforms. Therefore, this notation supports use cases needed for bottom-up and middle/topdown proteomics approaches and allows the encoding of highly modified proteins and peptides using a human and machine-readable string. ProForma 2.0 covers encoding protein modification names and accessions, cross-linking reagents including disulfides, glycans, modifications encoded using mass shifts and/or via chemical formulas, labile and C or N-terminal modifications, ambiguity in the modification position and representation of atomic isotopes, among other use cases. Notational conventions are based on public controlled vocabularies and ontologies. Detailed information about the notation and existing implementations are available at http://www.psidev.info/proforma and at the corresponding GitHub repository (https://github.com/HUPO-PSI/proforma).
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
