散射环境中共振粒子三维运动的宽场光瞬态成像
Widefield phototransient imaging for visualizing 3D motion of resonant particles in scattering environments
摘要：
在非特异性散射的存在下识别、可视化并最终跟踪动态移动的非荧光纳米颗粒是纳米和生命科学领域的一个长期挑战。在这项工作中，我们证明，我们最近开发的超快全息瞬态（UHT）显微镜是非常适合满足这一挑战。我们表明，UHT显微镜允许可靠地区分非共振，介电，从共振，金属，纳米粒子，基于光瞬态信号：在散射环境中的单粒子跟踪的先决条件。然后，我们证明了超高温显微镜的能力，全息定位在3D单粒子在大体积的视图。最终，我们结合这两个概念，同时跟踪几十个自由扩散的金纳米粒子，在一个110 × 110 × 110 $μ$ m的体积内的视图在每帧10毫秒的积分时间，同时记录他们的光瞬态信号。在这项工作中概述和验证的组合实验概念奠定了无背景3D单粒子跟踪应用或散射环境中的光谱学的基础，并立即适用于不同的系统，如活细胞和组织或支持的非均相催化剂。
英文摘要：
Identifying, visualising and ultimately tracking dynamically moving non-fluorescent nanoparticles in the presence of non-specific scattering is a long-standing challenge across the nano- and life-sciences. In this work we demonstrate that our recently developed ultrafast holographic transient (UHT) microscope is ideally suited for meeting this challenge. We show that UHT microscopy allows reliably distinguishing off-resonant, dielectric, from resonant, metallic, nanoparticles, based on the phototransient signal: a pre-requisite for single-particle tracking in scattering environments. We then demonstrate the capability of UHT microscopy to holographically localize in 3D single particles over large volumes of view. Ultimately, we combine the two concepts to simultaneously track several tens of freely diffusing gold nanoparticles, within a 110x110x110 $μ$m volume of view at an integration time of 10 ms per frame, while simultaneously recording their phototransient signals. The combined experimental concepts outlined and validated in this work lay the foundation for background-free 3D single-particle tracking applications or spectroscopy in scattering environments and are immediately applicable to systems as diverse as live cells and tissues or supported heterogeneous catalysts.
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