一种用于纤维素酶解过程原位高光谱成像的开放通道微流控膜装置
An Open-channel Microfluidic Membrane Device for In situ Hyperspectral mapping of enzymatic cellulose hydrolysis
摘要：
同步辐射红外超光谱显微镜是一种无标记和非侵入性的技术，非常适合原位成像的化学事件。它可以通过分子的特征振动模式来跟踪样品中感兴趣分子的时空分布。尽管近年来取得了巨大进展，但由于对环境控制的苛刻要求和水的强红外吸收，液体中生物材料中化学事件的红外高光谱成像仍然具有挑战性。在这里，我们报告了一个多相毛细管驱动的膜装置，用于无标记和实时的研究从刚毛藻纯化的藻纤维素酶解。
英文摘要：
Synchrotron infrared hyperspectral microscopy is a label-free and non-invasive technique well suited for imaging of chemical events in situ. It can track the spatial and temporal distributions of molecules of interests in a specimen in its native state by the molecule's characteristic vibrational modes. Despite tremendous progress made in recent years, IR hyperspectral imaging of chemical events in biomaterials in liquids remains challenging because of the demanding requirements on environmental control and strong infrared absorption of water. Here we report a multi-phase capillary-driven membrane device for label-free and real-time investigation of enzymatic deconstruction of algal cellulose purified from Cladophora aegagropila.
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