偶氮苯光异构探针细胞膜纳米粘度
Azobenzene photoisomerization probes cell membrane nanoviscosity
摘要：
细胞膜的粘度是影响小分子在细胞膜上和细胞膜内扩散的关键参数，并且与多种疾病有关。因此，在纳米尺度上定量测量膜粘度的可能性是非常感兴趣的。在这里，我们报告了一个完整的调查的两亲性膜靶向偶氮苯（ZIAPIN 2），我们验证其作为细胞膜的粘度探针。我们利用ZIAPIN 2的反-顺光异构化，开发了一种分子粘度计，并采用时间分辨荧光光谱法评估大肠杆菌细菌膜的粘度。ZIAPIN 2在E.大肠杆菌悬浮液正确地表明，膜粘度随着样品加热而降低。我们的结果报告了活大肠杆菌的膜粘度值。大肠杆菌细胞从10到5 cP，将温度从22 °C增加到40 °C。
英文摘要：
The viscosity of cell membranes is a crucial parameter that affects the diffusion of small molecules both across and within the lipidic membrane and that is related to several diseases. Therefore, the possibility to measure quantitatively membrane viscosity on the nanoscale is of great interest. Here, we report a complete investigation of the photophysics of an amphiphilic membrane-targeted azobenzene (ZIAPIN2) and we validate its use as viscosity probe for cell membranes. We exploit ZIAPIN2 the trans-cis photoisomerization to develop a molecular viscometer and to assess the viscosity of Escherichia coli bacteria membranes employing time-resolved fluorescence spectroscopy. Lifetime measurements of ZIAPIN2 in E. coli bacteria suspensions correctly indicate that membrane viscosity decreases as the samples were heated up. Our results report a membrane viscosity value in live E. coli cells going from 10 to 5 cP, increasing the temperature from 22 °C up to 40 °C.
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