利用偶氮苯光控制使蛋白质运动
Using Azobenzene Photocontrol to Set Proteins in Motion
摘要：
利用偶氮苯光开关控制蛋白质的活性是操纵其生物学功能的有力工具。在光的帮助下，人们可以改变例如结合亲和力，控制变构或调节复杂的生物过程。此外，由于其固有的快速光异构化，偶氮苯光开关可以作为触发器启动平衡过程。因此，这种活动的转换启动了一系列构象事件，这些事件只能用时间分辨的方法来访问。在这篇综述中，我们将展示如何将偶氮苯光开关的潜力与瞬态光谱技术相结合，有助于揭示事件的顺序，并提供实时生物分子相互作用的实验观察。这将最终帮助我们了解蛋白质如何适应，适应和重新调整其结构以响应传入的信号，并将完成我们对蛋白质动力学特征的了解。
英文摘要：
Controlling the activity of proteins with azobenzene photoswitches is a potent tool for manipulating their biological function. With the help of light, one can change e.g. binding affinities, control allostery or temper with complex biological processes. Additionally, due to their intrinsically fast photoisomerisation, azobenzene photoswitches can serve as triggers to initiate out-of-equilibrium processes. Such switching of the activity, therefore, initiates a cascade of conformational events, which can only be accessed with time-resolved methods. In this Review, we will show how combining the potency of azobenzene photoswitching with transient spectroscopic techniques helps to disclose the order of events and provide an experimental observation of biomolecular interactions in real-time. This will ultimately help us to understand how proteins accommodate, adapt and readjust their structure to answer an incoming signal and it will complete our knowledge of the dynamical character of proteins.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
