利用运动细胞快速测量微通道声电泳中的局部压力幅值
Rapid measurement of the local pressure amplitude in microchannel acoustophoresis using motile cells
摘要：
声学微流体技术（或声流体技术）提供了一种非接触和无标记的手段来操纵和询问生物颗粒。由于其生物相容性和精确性，声流体方法在生物医学研究的各个领域实现了创新。未来的突破将依赖于将这些技术从学术实验室转化为临床和工业环境。在这里，器件性能的准确表征和标准化至关重要。需要通用的、快速的和广泛可访问的性能量化。我们提出了一个领域的定量方法，利用运动的莱茵衣藻藻细胞。我们以前报道的定性映射声场使用活的microswimmer作为主动探头。在本研究中，我们扩展我们的方法，以实现具有挑战性的定量原位测量的声学能量密度。C. Reinhardtii细胞在施加的力场中连续游动，并随着力场的变化而动态地重新分布。这种行为允许准确和完整的实时性能监测，可以很容易地在声流体和更广泛的微流体研究社区中应用和采用。此外，该方法仅依赖于标准的明场显微镜，可以在几分钟内评估多种条件下的视野。我们对传统的被动粒子跟踪的方法进行基准测试，达到1%内的协议，从0到100 J m-3（0到~1 MPa）的场强。
英文摘要：
Acoustic microfluidics (or acoustofluidics) provides a non-contact and label-free means to manipulate and interrogate bioparticles. Owing to their biocompatibility and precision, acoustofluidic approaches have enabled innovations in various areas of biomedical research. Future breakthroughs will rely on translation of these techniques from academic labs to clinical and industrial settings. Here, accurate characterization and standardization of device performance is crucial. Versatile, rapid, and widely accessible performance quantification is needed. We propose a field quantification method using motile Chlamydomonas reinhardtii algae cells. We previously reported qualitative mapping of acoustic fields using living microswimmers as active probes. In the present study, we extend our approach to achieve the challenging quantitative in situ measurement of the acoustic energy density. C. reinhardtii cells continuously swim in an imposed force field and dynamically redistribute as the field changes. This behavior allows accurate and complete, real-time performance monitoring, which can be easily applied and adopted within the acoustofluidics and broader microfluidics research communities. Additionally, the approach relies only on standard bright-field microscopy to assess the field under numerous conditions within minutes. We benchmark the method against conventional passive-particle tracking, achieving agreement within 1 % for field strengths from 0 to 100 J m-3 (0 to ~1 MPa).
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