用明场显微镜探测各向异性小胶体颗粒的旋转平移扩散
Probing roto-translational diffusion of small anisotropic colloidal particles with a bright-field microscope
摘要：
软材料和生物材料通常由基本成分组成，在微观尺度上表现出不断的旋转平移运动。当颗粒尺寸变得小于显微镜分辨率时，用明场显微镜跟踪这种运动变得越来越具有挑战性，这是经常遇到的情况。在这里，我们展示了平方梯度微分动态显微镜（SG-DDM）作为一种工具，成功地使用明场显微镜提取小的各向异性胶体颗粒的旋转平移动力学，其旋转运动不能准确地跟踪在直接空间。我们提供了分析的理由和实验证明的方法，成功地应用于花生状颗粒的水悬浮液。
英文摘要：
Soft and biological materials are often composed of elementary constituents exhibiting an incessant roto-translational motion at the microscopic scale. Tracking this motion with a bright-field microscope becomes increasingly challenging when the particle size becomes smaller than the microscope resolution, a case which is frequently encountered. Here we demonstrate Squared-Gradient Differential Dynamic Microscopy (SG-DDM) as a tool to successfully use bright-field microscopy to extract the roto-translational dynamics of small anisotropic colloidal particles, whose rotational motion cannot be tracked accurately in direct space. We provide analytical justification and experimental demonstration of the method by successful application to an aqueous suspension of peanut-shaped particles.
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