ExpressInHost：用于在宿主微生物中表达重组蛋白的密码子调整工具
ExpressInHost: A codon tuning tool for the expression of recombinant proteins in host microorganisms
摘要：
RAGINHost（https：//gitlab.com/a.raguin/expressinhost）是基于GTK/C++的用户友好的图形界面，其允许调整mRNA的密码子序列以用于宿主微生物中的重组蛋白表达。异源基因表达在生物技术公司和学术研究实验室中广泛实施。然而，重组蛋白的表达可能具有挑战性。一方面，使翻译速度最大化是重要的，特别是在与生物技术公司相关的可扩展的生产过程中，但另一方面，溶解性问题通常会因此而出现，因为翻译“暂停”可能是允许新生多肽链适当折叠的关键。为了应对这一挑战，我们开发了一种软件，该软件提供了三种不同的模式来使用遗传密码冗余调整密码子序列。实施的调整策略考虑到宿主和天然生物体的特定tRNA资源。它们平衡了快速翻译和天然速度模仿，这对于允许适当的蛋白质折叠可能是重要的，从而避免蛋白质溶解度问题。
英文摘要：
ExpressInHost (https://gitlab.com/a.raguin/expressinhost) is a GTK/C++ based user friendly graphical interface that allows tuning the codon sequence of an mRNA for recombinant protein expression in a host microorganism. Heterologous gene expression is widely implemented in biotechnology companies and academic research laboratories. However, expression of recombinant proteins can be challenging. On the one hand, maximising translation speed is important, especially in scalable production processes relevant to biotechnology companies, but on the other hand, solubility problems often arise as a consequence, since translation "pauses" might be key to allow the nascent polypeptide chain to fold appropriately. To address this challenge, we have developed a software that offers three distinct modes to tune codon sequences using the genetic code redundancy. The tuning strategies implemented take into account the specific tRNA resources of the host and that of the native organism. They balance rapid translation and native speed mimicking, which might be important to allow proper protein folding, thereby avoiding protein solubility problems.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
