RNA聚合中密度和伸长速度相关的误差校正
Density- and elongation speed-dependent error correction in RNA polymerization
摘要：
RNA聚合酶的回溯（RNAP）是DNA转录过程中一种重要的暂停机制，是提高转录保真度的纠错过程的一部分。我们对RNA聚合酶的回溯机制进行了建模，这通常发生在聚合酶试图掺入错配的核苷酸三磷酸时。先前的模型已经做出了简化的假设，例如忽略回溯聚合酶后面的拖尾聚合酶或假设拖尾聚合酶是固定的。我们推导出精确的解析解的随机模型，其中包括本地相互作用的RNAP明确显示如何尾随RNAP影响的概率，错误被纠正或纳入领先的回溯RNAP。我们还提供了两个相关的方法来计算平均时间错误校正或合并给定的初始本地RNAP配置。
英文摘要：
Backtracking of RNA polymerase (RNAP) is an important pausing mechanism during DNA transcription that is part of the error correction process that enhances transcription fidelity. We model the backtracking mechanism of RNA polymerase, which usually happens when the polymerase tries to incorporate a mismatched nucleotide triphosphate. Previous models have made simplifying assumptions such as neglecting the trailing polymerase behind the backtracking polymerase or assuming that the trailing polymerase is stationary. We derive exact analytic solutions of a stochastic model that includes locally interacting RNAPs by explicitly showing how a trailing RNAP influences the probability that an error is corrected or incorporated by the leading backtracking RNAP. We also provide two related methods for computing the mean times to error correction or incorporation given an initial local RNAP configuration.
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