三维傅里叶变换光散射重建单个粒子的扩展角分辨光散射
3D Fourier transformation light scattering for reconstructing extend angled resolved light scattering of individual particles
摘要:
我们代表三维傅里叶变换光散射,一种方法来重建角分辨光散射(ARLS)与扩展的角度范围从个别球形物体。为了克服光学系统的物理数值孔径所决定的角度限制,在不同的照明角度下测量样品散射的光学光场,然后通过旋转散射光信号将其合成到傅立叶空间中与法向照明相对应的埃瓦尔德球上。该方法将ARLS光谱的角度范围扩展到90度以上,超出了前向光学测量的限制。扩展的散射光场在3D和相应的ARLS光谱的个别微观聚苯乙烯珠,和蛋白质液滴表示。
英文摘要:
We represent three-dimensional Fourier transform light scattering, a method to reconstruct angle-resolved light scattering (ARLS) with extended angle-range from individual spherical objects. To overcome the angle limitation determined by the physical numerical aperture of an optical system, the optical light fields scattered from a sample are measured with various illumination angles, and then synthesized onto the Ewald Sphere corresponding to the normal illumination in Fourier space by rotating the scattered light signals. The method extends the angle range of the ARLS spectra beyond 90 degree, beyond the limit of forward optical measurements. Extended scattered light fields in 3D and corresponding ARLS spectra of individual microscopic polystyrene beads, and protein droplets are represented.
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