冷冻液体的原子探针分析现状及发展方向
Status and direction of atom probe analysis of frozen liquids
摘要：
通过电子显微镜对液体和低温生物材料进行成像最近已经通过用于样品制备的创新方法和用于低温启用电子显微镜（cryo-EM）的优化仪器的快速开发而实现。然而，Cryo-EM通常缺乏先进的分析能力，特别是对于轻元素。随着冷冻湿标本制备方案的发展，原子探针断层扫描（APT）可以有利地补充冷冻EM获得的见解。在这里，我们报告了不同的方法，最近提出的，使相对较大的体积的冷冻液体的分析，无论是从一个平面基板或断裂表面的电线。这两种仪器都可以分析由纯水、纯重水和水溶液组成的几微米厚的水冰层。我们讨论这两种方法的优点，并在这一领域的进一步发展的前景。初步结果提出了许多问题，部分涉及物理基础场蒸发。我们讨论了这些方面，并提出了一些关于APT分析冷冻液体的挑战。
英文摘要：
Imaging of liquids and cryogenic biological materials by electron microscopy has been recently enabled by innovative approaches for specimen preparation and the fast development of optimised instruments for cryo-enabled electron microscopy (cryo-EM). Yet, Cryo-EM typically lacks advanced analytical capabilities, in particular for light elements. With the development of protocols for frozen wet specimen preparation, atom probe tomography (APT) could advantageously complement insights gained by cryo-EM. Here, we report on different approaches that have been recently proposed to enable the analysis of relatively large volumes of frozen liquids from either a flat substrate or the fractured surface of a wire. Both allowed for analysing water ice layers which are several microns thick consisting of pure water, pure heavy-water and aqueous solutions. We discuss the merits of both approaches, and prospects for further developments in this area. Preliminary results raise numerous questions, in part concerning the physics underpinning field evaporation. We discuss these aspects and lay out some of the challenges regarding the APT analysis of frozen liquids.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
