H. sapiens和恶性疟原虫，预测其与HLA-A *24：02强结合并与SARS-CoV-2肽同源
Peptides of H. sapiens and P. falciparum that are predicted to bind strongly to HLA-A*24:02 and homologous to a SARS-CoV-2 peptide
摘要：
目的：本研究旨在寻找SARS-CoV-2和疟原虫物种在具有某些HLA血清型的个体中的共同致病性。方法：1-）通过限制搜索感染人类的疟原虫物种来执行SARS-CoV-2的Tblastx搜索。2-)用blastp搜索相应生物体蛋白质组中的比对序列。3-)进行鉴定的SARS-CoV-2肽与MHC I类超型代表的结合预测。4-)通过限制对人和疟原虫物种的搜索来进行与所鉴定的HLA等位基因强结合的预测表位的Blastp搜索。5-)在结果中发现与预测表位具有至少60%同一性的肽。6-)预测其中与相同HLA等位基因强烈结合的肽。7-)通过限制对人的搜索重复步骤4，对于通过在步骤4中限制对疟原虫物种的搜索而获得的肽。8-)执行步骤5和6，结果为7。结果：SARS-CoV-2的CFLGYFCTCYFGLFC肽与间日疟原虫的同一性最高。其GYFCTCYFGLF和YFCTCYFGLF部分被预测与HLA-A*24：02强烈结合。仅获得与YFCTCYFGLF同源的肽的结果如下：YYCARRFGLF、YYCHCPFGVF和YYCQQYFFLF是人类蛋白质组中潜在的HLA-A*24：02表位。在疟原虫种蛋白质组中对HLA-A*24：02具有强结合亲和力的FFYTFYFELF、YFVACLFILF和YFPTITFHLF肽中，只有恶性疟原虫的FFYTFYFELF与人蛋白质组中潜在的HLA-A*24：02表位YFYLFSLELF同源。结论：对具有相似序列和与HLA-A*24：02的强结合亲和力的鉴定肽的免疫应答可能导致具有HLA-A*24：02血清型的个体在感染SARS-CoV-2或恶性疟原虫后的自身免疫应答风险。
英文摘要：
Aim: This study is looking for a common pathogenicity between SARS-CoV-2 and plasmodium species, in individuals with certain HLA serotypes. Methods: 1-) Tblastx searches of SARS-CoV-2 are performed by limiting searches to plasmodium species that infect human. 2-) Aligned sequences in the respective organisms' proteomes are searched with blastp. 3-) Binding predictions of the identified SARS-CoV-2 peptide to MHC class I supertype representatives are performed. 4-) Blastp searches of predicted-epitopes that bind strongly to the identified HLA allele are performed by limiting searches to human and to the plasmodium species. 5-) Peptides with minimum 60 % identity to the predicted-epitopes are found in results. 6-) Peptides among those, which bind strongly to the same HLA allele, are predicted. 7-) Step-4 is repeated by limiting searches to human, for peptides sourced by limiting searches to plasmodium species at step-4. 8-) Step-5 and 6 are performed with results of 7. Results: CFLGYFCTCYFGLFC peptide of SARS-CoV-2 has the highest identity to P. vivax. Its GYFCTCYFGLF and YFCTCYFGLF parts are predicted to bind strongly to HLA-A*24:02. Results obtained only for peptides homologous to YFCTCYFGLF, as follows: YYCARRFGLF, YYCHCPFGVF, and YYCQQYFFLF are potential HLA-A*24:02 epitopes in the human proteome. Among FFYTFYFELF, YFVACLFILF, and YFPTITFHLF peptides in the plasmodium species' proteomes with strong binding affinity to HLA-A*24:02, only FFYTFYFELF of P. falciparum is homologous to the potential HLA-A*24:02 epitope YFYLFSLELF in the human proteome. Conclusion: Immune responses to the identified-peptides with similar sequences and strong binding affinities to HLA-A*24:02 may lead to autoimmune response risk in individuals with HLA-A*24:02 serotypes, upon getting infected with SARS-CoV-2 or P. falciparum.
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