16 SrDNA高通量测序对塞浦路斯驴奶细菌多样性的研究
Investigation of the Cyprus donkey milk bacterial diversity by 16SrDNA high-throughput sequencing in a Cyprus donkey farm
摘要：
由于其声称的功能和营养特性，特别是对于敏感人群，如对牛奶蛋白过敏的婴儿，对驴奶的兴趣在全球范围内不断增长。目前的研究旨在使用基于培养的高通量测序（HTS）技术评估塞浦路斯驴场生产的驴奶的微生物质量。基于培养的微生物分析显示微生物计数非常低，而重要的食源性病原体在任何样品中均未检出。此外，HTS被应用于驴乳样品的细菌群落特征。驴奶主要由以下细菌组成：革兰氏阴性变形菌，包括鞘氨醇单胞菌、中慢生根瘤假单胞菌和不动杆菌;乳酸菌，包括乳杆菌和链球菌;形成梭菌的内生孢子;以及环境属黄杆菌和罗尔斯通氏菌，检测到的相对丰度较低。这项研究的结果支持了现有的发现，即驴奶主要含有革兰氏阴性细菌。此外，它提出了关于以下方面的贡献的问题：a）抗菌剂（即溶菌酶、肽）在塑造微生物群落中的作用，b）细菌微生物群对驴奶的功能价值的作用。
英文摘要：
The interest in milk originating from donkeys is growing worldwide due to its claimed functional and nutritional properties, especially for sensitive population groups, such as infants with cow milk protein allergy. The current study aimed to assess the microbiological quality of donkey milk produced in a donkey farm in Cyprus using cultured-based and high-throughput sequencing (HTS) techniques. The culture-based microbiological analysis showed very low microbial counts, while important food-borne pathogens were not detected in any sample. In addition, HTS was applied to characterize the bacterial communities of donkey milk samples. Donkey milk was mostly comprised of: Gram-negative Proteobacteria, including Sphingomonas, Pseudomonas Mesorhizobium and Acinetobacter; lactic acid bacteria, including Lactobacillus and Streptococcus; the endospores forming Clostridium; and the environmental genera Flavobacterium and Ralstonia, detected in lower relative abundances. The results of the study support existing findings that donkey milk contains mostly Gram-negative bacteria. Moreover, it raises questions regarding the contribution: a) of antimicrobial agents (i.e. lysozyme, peptides) in shaping the microbial communities and b) of the bacterial microbiota to the functional value of donkey milk.
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