基于周期性浓度极化的生物分子预浓缩物的形成及其生物传感性能
Periodic Concentration-Polarization-Based Formation of a Biomolecule Preconcentrate for Enhanced Biosensing
摘要：
基于离子浓度极化（CP）的生物分子预富集是一种提高目标生物分子检测灵敏度的成熟方法。然而，所形成的预浓缩生物分子塞快速扫过表面固定的抗体，导致捕获剂和分析物之间的短期重叠，以及随后的次优结合。为了克服这一点，我们设计了一种设置，允许通过激活CP预定的开/关间隔来周期性地形成预浓缩的生物分子塞。这项工作证明了循环CP致动的可行性，并优化了所需的扫描条件，以获得最大的保留时间的预浓缩塞在所需的感测区域和增强的检测灵敏度。这种方法能够有效地预浓缩不同的分析物，并成功地提高免疫测定的灵敏度，强调其在免疫测定服务于临床和食品检测行业的潜力。
英文摘要：
Ionic concentration-polarization (CP)-based biomolecule preconcentration is an established method for enhancing the detection sensitivity of target biomolecules. However, the formed preconcentrated biomolecule plug rapidly sweeps over the surface-immobilized antibodies, resulting in a short-term overlap between the capture agent and the analyte, and subsequently suboptimal binding. To overcome this, we designed a setup allowing for periodic formation of a preconcentrated biomolecule plug by activating the CP for predetermined on/off intervals. This work demonstrated the feasibility of the cyclic CP actuation and optimized the sweeping conditions required to obtain maximum retention time of a preconcentrated plug over a desired sensing region and enhanced detection sensitivity. The ability of this method to efficiently preconcentrate different analytes and to successfully increase immunoassay sensitivity, underscore its potential in immunoassays serving the clinical and food testing industries.
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