定向冻融条件下纳米壳涂层细胞的预熔定向界面设计
Premelting-directed interfacial design of nanoshell-coated cells in directional freezing and thawing
摘要：
冷冻界面与软细胞的相互作用是低温保存的核心问题。然而，大多数现有的冷冻保存方法面临着诸如复杂的处理和差的可控性的挑战。在这里，我们报告了一种新的，基于纳米技术的方法来调节冰和细胞之间的界面相互作用，通过预融理论。通过可控地修饰细胞表面的生物相容性纳米壳的界面设计，可以改变细胞与冰之间的有效Hamaker常数。进一步调节细胞与冰之间的预融膜厚度，使包被细胞在热梯度中发生定向迁移，实现包被细胞定向解冻。冷冻包被细胞死亡率的降低表明，冻融过程中的预融调制可以有效地保护细胞免受冷冻过程中冰形成和解冻过程中冰重结晶的机械损伤。
英文摘要：
Freezing interface interacting with soft cells is a core issue in cryopreservation. However, most of existing cryopreservation approaches face challenges such as complex processing and poor controllability. Herein we report a new, nanotechnology-based method to modulate interfacial interactions between ice and cells via the premelting theory. Through the interfacial design by controllably modifying biocompatible nanoshells on the surface of cells, the effective Hamaker constant between cells and ice can be modified. The thickness of premelted films between cells and ice is further regulated, and directional migration of coated cells occurs in a thermal gradient to achieve directional thawing of coated cells. The decreased mortality of freezing coated cells suggests the premelting modulation in freezing and thawing can effectively protect cells from mechanical damage of ice formation during freezing and ice recrystallization during thawing.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
