分析健康和疾病中的核糖体重塑
Analyzing ribosome remodeling in health and disease
摘要：
监管在很大程度上尚未探索，部分原因是方法上的限制。事实上，我们审查的证据表明，常用的方法，如转录组学，是不够的，因为编码核糖体蛋白（RP）的mRNA的变异性不一定对应于RP的变异性。因此，核糖体的蛋白质重塑应该通过允许直接定量RP的方法来研究，理想的是分离的核糖体。我们回顾了此类方法，重点关注质谱法，并强调方法特定的偏差和控制这些偏差的方法。我们认为，使用多种互补的方法可以帮助减少将可重复的系统偏差解释为核糖体重塑证据的危险。
英文摘要：
regulation largely unexplored, in part due to methodological limitations. Indeed, we review evidence demonstrating that commonly used methods, such as transcriptomics, are inadequate because the variability in mRNAs coding for ribosomal proteins (RP) does not necessarily correspond to RP variability. Thus protein remodeling of ribosomes should be investigated by methods that allow direct quantification of RPs, ideally of isolated ribosomes. We review such methods, focusing on mass spectrometry and emphasizing method-specific biases and approaches to control these biases. We argue that using multiple complementary methods can help reduce the danger of interpreting reproducible systematic biases as evidence for ribosome remodeling.
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