用于探测多尺度蛋白质力学和组装的光镊方法
Optical tweezers approaches for probing multiscale protein mechanics and assembly
摘要：
单个蛋白质构建块的多步组装是形成细胞内外基本高阶结构的关键。光镊是一种非常适合研究这些结构在各种尺寸尺度下的力学和动力学的技术。在这篇小型综述中，我们重点介绍了使用光镊研究蛋白质组装和力学的实验，重点是细胞外基质蛋白质胶原。这些例子展示了如何使用光镊来研究从单分子水平到纤维到蛋白质网络的长度尺度上的力学。我们讨论了在实验设计和解释的挑战，与其他实验方式整合的机会，以及光镊在蛋白质力学和组装当前问题的应用。
英文摘要：
Multi-step assembly of individual protein building blocks is key to the formation of essential higher-order structures inside and outside of cells. Optical tweezers is a technique well suited to investigate the mechanics and dynamics of these structures at a variety of size scales. In this mini-review, we highlight experiments that have used optical tweezers to investigate protein assembly and mechanics, with a focus on the extracellular matrix protein collagen. These examples demonstrate how optical tweezers can be used to study mechanics across length scales, ranging from the single-molecule level to fibrils to protein networks. We discuss challenges in experimental design and interpretation, opportunities for integration with other experimental modalities, and applications of optical tweezers to current questions in protein mechanics and assembly.
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