周期性应变的肺泡拟态及其对细胞层形成的影响
Alveolar mimics with periodic strain and its effect on the cell layer formation
摘要：
我们报告的发展，一个新的模型肺泡空气组织界面上的芯片。该模型由微框架和微流控装置支持的悬浮六边形单层明胶纳米纤维阵列的补丁集成。通过施加200-1000 Pa范围内的空气压力，悬浮单分子层在空气-液体界面处变形至40-80 μ m的中心位移。相对于500 μ m的单层直径，该位移对应于与肺泡中的生理应变范围一致的2-10%的线性应变。A549细胞在单层上培养1-3天，在模型中显示出活力。我们在1小时内以0.2 Hz的频率对细胞施加5%的周期性应变。我们发现细胞与纳米纤维强烈耦合，但应变降低了耦合并诱导了肌动蛋白细胞骨架的重塑，从而导致更好的组织形成。我们的模型可以作为肺部研究的通用工具，如吸入毒理学和治疗。
英文摘要：
We report on the development of a new model of alveolar air-tissue interface on a chip. The model consists of an array of suspended hexagonal monolayers of gelatin nanofibers supported by microframes and a microfluidic device for the patch integration. The suspended monolayers are deformed to a central displacement of 40-80 um at the air-liquid interface by application of air pressure in the range of 200-1000 Pa. With respect to the diameter of the monolayers that is 500 um, this displacement corresponds to a linear strain of 2-10% in agreement with the physiological strain range in the lung alveoli. The culture of A549 cells on the monolayers for an incubation time 1-3 days showed viability in the model. We exerted a periodic strain of 5% at a frequency of 0.2 Hz during 1 hour to the cells. We found that the cells were strongly coupled to the nanofibers, but the strain reduced the coupling and induced remodeling of the actin cytoskeleton, which led to a better tissue formation. Our model can serve as a versatile tool in lung investigations such as in inhalation toxicology and therapy.
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