电纺纳米金刚石-丝素蛋白膜：生物传感和伤口愈合应用的多功能平台
Electrospun nanodiamond-silk fibroin membranes: a multifunctional platform for biosensing and wound healing applications
摘要：
下一代伤口护理技术能够诊断伤口参数，促进健康细胞生长，减少病原性感染，非侵入性将为患者提供更好的护理标准和加速伤口修复。温度是慢性伤口特异性的指示生物标志物之一。这项工作报告了一个混合的，多功能的光学平台：纳米金刚石丝膜作为生物启发敷料能够温度传感和伤口愈合。通过静电纺丝制备杂化材料，并形成具有高孔隙率的亚微米纤维膜。丝纤维能够补偿伤口部位细胞外基质的缺乏，支持伤口愈合。纳米金刚石（ND）中带负电荷的氮空位（NV-）色心表现出光学检测磁共振（ODMR）特性，并充当荧光纳米级温度计，能够感测与伤口中感染或炎症相关的温度变化，而无需物理移除敷料。我们的研究结果表明，ND的存在下，在混合ND-丝膜提高丝纤维的热稳定性。嵌在丝纤维中的ND中的NV-色心表现出良好的荧光和ODMR保留。使用NV-中心作为荧光纳米级温度计，我们在细胞培养的ND-丝膜上实现了在一系列温度下的温度感测，包括生物学相关的温度窗口。增强的NV-中心的温度灵敏度的观察到的混合。在鼠伤口愈合模型中进一步体内测试膜，并证明生物相容性和与对照伤口等同的伤口闭合率。此外，混合ND-丝膜显示出对革兰氏阴性铜绿假单胞菌和大肠杆菌的选择性抑制和杀生物倾向。
英文摘要：
Next generation wound care technology capable of diagnosing wound parameters, promoting healthy cell growth and reducing pathogenic infections noninvasively will provide patients with an improved standard of care and an accelerated wound repair. Temperature is one of the indicating biomarkers specific to chronic wounds. This work reports a hybrid, multifunctional optical platform: nanodiamond-silk membranes as bioinspired dressings capable of temperature sensing and wound healing. The hybrid was fabricated through electrospinning and formed sub-micron fibrous membranes with high porosity. The silk fibres are capable of compensating for the lack of extracellular matrix at the wound site, supporting the wound healing. The negatively charged nitrogen vacancy (NV-) color centres in nanodiamonds (NDs) exhibit optically detected magnetic resonance (ODMR) properties and act as fluorescent nanoscale thermometers, capable of sensing temperature variations associated to the presence of infection or inflammation in a wound, without physically removing the dressing. Our results show that the presence of NDs in the hybrid ND-silk membranes improve the thermal stability of silk fibres. The NV- color centres in NDs embedded in silk fibres exhibit well-retained fluorescent and ODMR. Using the NV- centres as fluorescent nanoscale thermometers, we achieved temperature sensing at a range of temperatures, including the biologically relevant temperature window, on cell-cultured ND-silk membranes. Enhancement in the temperature sensitivity of the NV- centres was observed for the hybrids. The membranes were further tested in vivo in a murine wound healing model and demonstrated biocompatibility and equivalent wound closure rates as the control wounds. Additionally, the hybrid ND-silk membranes showed selective antifouling and biocidal propensity toward Gram-negative Pseudomonas aeruginosa and Escherichia coli.
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