高尔基体中的聚糖加工--最佳信息编码及对池数和酶特异性的限制
Glycan processing in the Golgi -- optimal information coding and constraints on cisternal number and enzyme specificity
摘要：
许多在高尔基池中经历连续酶促修饰的蛋白质作为细胞身份标记物展示在质膜上。被修饰的蛋白质，称为聚糖，代表一种分子密码。该聚糖编码的保真度通过聚糖合成机制如何准确地实现特定细胞类型和小生境的所需靶聚糖分布来测量。在本文中，我们定量分析的数量之间的权衡池和酶的数量和特异性，以合成一个规定的目标聚糖分布的一定的复杂性。我们发现，合成复杂的分布，如在真实的细胞中观察到的，一个需要有多个池和精确的酶分区在高尔基体。此外，对于固定数量的酶和池，存在最佳水平的酶特异性，其以高保真度实现靶分布。我们的研究结果表明，目标聚糖分布的复杂性如何对高尔基体池的数量和酶的特异性的功能限制。
英文摘要：
Many proteins that undergo sequential enzymatic modification in the Golgi cisternae are displayed at the plasma membrane as cell identity markers. The modified proteins, called glycans, represent a molecular code. The fidelity of this glycan code is measured by how accurately the glycan synthesis machinery realises the desired target glycan distribution for a particular cell type and niche. In this paper, we quantitatively analyse the tradeoffs between the number of cisternae and the number and specificity of enzymes, in order to synthesize a prescribed target glycan distribution of a certain complexity. We find that to synthesize complex distributions, such as those observed in real cells, one needs to have multiple cisternae and precise enzyme partitioning in the Golgi. Additionally, for fixed number of enzymes and cisternae, there is an optimal level of specificity of enzymes that achieves the target distribution with high fidelity. Our results show how the complexity of the target glycan distribution places functional constraints on the Golgi cisternal number and enzyme specificity.
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