确定细胞外囊泡大小、浓度、折射率和表位丰度的参考材料
Towards defining reference materials for extracellular vesicle size, concentration, refractive index and epitope abundance
摘要：
细胞外囊泡（EV）的准确表征对于探索其诊断和治疗应用至关重要。随着EV研究领域的发展，用于表征它们的技术也在发展。为了使这些技术标准化，需要开发参考材料。这项工作始于英国伯明翰的ISEV 2017生物标志物研讨会，并在比利时根特的ISEV 2019标准化研讨会期间进行了进一步讨论，旨在阐明需要哪些参考材料，以及目前可用于标准化常用的分析平台，以表征EV大小，浓度，折射率和表位表达。由于它们的主要用途，特别关注的是光学方法纳米粒子跟踪分析和流式细胞术。
英文摘要：
Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials is required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and with further discussion during the ISEV 2019 Standardization Workshop in Ghent, Belgium, sets out to elucidate which reference materials are required and which are currently available to standardize commonly used analysis platforms for characterizing EV size, concentration, refractive index, and epitope expression. Due to their predominant use, a particular focus is placed on the optical methods nanoparticle tracking analysis and flow cytometry.
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