固定剂对RP-CARS显微镜观察髓鞘分子有序性的影响
Effects of fixatives on myelin molecular order probed with RP-CARS microscopy
摘要：
当实时成像不可行时，样品固定允许保存生物样品的超微结构以用于随后的显微镜分析。这个过程可以用各种方法进行，每种方法对样品的生物结构有不同的影响。虽然这些变化的特点是使用传统的显微镜，很少的信息是可用的固定剂对生物组织的空间分子取向的影响。我们解决了这个问题，采用旋转偏振相干反斯托克斯拉曼散射（RP-CARS）显微镜，研究不同的固定剂对髓鞘亚微米分子顺序和微米形态的影响。RP-CARS是一种源自CARS显微镜的新技术，它允许探测分子键的空间取向，同时保持CARS显微镜固有的化学选择性。通过表征的固定程序的影响，目前的工作代表了一个有用的指导选择的最佳固定技术（S），特别是偏振分辨CARS显微镜。最后，我们表明，多聚甲醛和戊二醛的组合可以有效地用作RP-CARS显微镜的固定剂，只要考虑到对分子空间分布的影响，在这里表征。
英文摘要：
When live imaging is not feasible, sample fixation allows preserving the ultrastructure of biological samples for subsequent microscopy analysis. This process could be performed with various methods, each one affecting differently the biological structure of the sample. While these alterations were well-characterized using traditional microscopy, little information is available about the effects of the fixatives on the spatial molecular orientation of the biological tissue. We tackled this issue by employing Rotating-Polarization Coherent Anti-Stokes Raman Scattering (RP-CARS) microscopy to study the effects of different fixatives on the myelin sub-micrometric molecular order and micrometric morphology. RP-CARS is a novel technique derived from CARS microscopy that allows probing spatial orientation of molecular bonds while maintaining the intrinsic chemical selectivity of CARS microscopy. By characterizing the effects of the fixation procedures, the present work represents a useful guide for the choice of the best fixation technique(s), in particular for polarisation-resolved CARS microscopy. Finally, we show that the combination of paraformaldehyde and glutaraldehyde can be effectively employed as a fixative for RP-CARS microscopy, as long as the effects on the molecular spatial distribution, here characterized, are taken into account.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
