扫描显微OCT中的动态对比
Dynamic contrast in scanning microscopic OCT
AI总结：
本文提出在扫描频域OCT中实现动态对比，利用细胞结构ATP依赖性运动增强散射对比，以更快、更高效地获得类似组织学的深度分辨图像，为未染色组织分析提供新工具。
摘要：
虽然光学相干断层扫描（OCT）可提供低至1微米的分辨率，但由于缺乏散射对比，其在可视化细胞结构方面存在困难。通过评估信号波动，使用时域全场OCT（FF-OCT）已证明可实现显著的对比度增强，从而使细胞和亚细胞结构可见。动态OCT信号的推测原因是细胞结构在亚微米范围内的ATP依赖性运动，这提供了类似组织学的对比。在此，我们展示了使用扫描频域OCT（FD-OCT）实现动态对比。鉴于固有的截面成像几何结构，扫描FD-OCT可提供跨组织层的深度分辨图像，这一视角在组织病理学中为人所知，且比FF-OCT更快、更高效。较短的采集时间和断层深度切片均降低了动态对比对整体组织运动伪影的敏感性，并简化了后处理中的校正。动态对比的实现使显微FD-OCT成为未染色组织组织学分析的有前景工具。
英文摘要：
While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures visible. The putative cause of the dynamic OCT signal is ATP-dependent motion of cellular structures in a sub-micrometer range, which provides histology-like contrast. Here we demonstrate dynamic contrast with a scanning frequency-domain OCT (FD-OCT). Given the inherent sectional imaging geometry, scanning FD-OCT provides depth-resolved images across tissue layers, a perspective known from histopathology, much faster and more efficiently than FF-OCT. Both, shorter acquisition times and tomographic depth-sectioning reduce the sensitivity of dynamic contrast for bulk tissue motion artifacts and simplify their correction in post-processing. The implementation of dynamic contrast makes microscopic FD-OCT a promising tool for histological analysis of unstained tissues.
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