Lensless microscopy with coherent or partially coherent light sources is a well known imaging technique, commonly referred as digital in-line holographic microscopy. In the established methods, both the spatial and temporal coherence of light play a crucial role in determining the resolution of reconstructed object. We report lensless microscopy with a spatially extended white LED, a light source of low spatial and very low temporal coherence. The wave-field propagation between two parallel planes can be obtained using a convolution operation, where the convolution kernel depends on the object-sensor distance and the characteristics of the light source. For a light source of unknown characteristics, this kernel is an unknown function. In the proposed reconstruction method, we decompose an unknown convolution kernel of very large size (128 X 128) into a small unknown light-source-specific kernel (size 9 X 9) and a known light-source-independent kernel (size 128 X 128). This drastically reduces the number of parameters to be estimated at the system identification step, which has been performed here by one time imaging of the known microscopic objects. Final unknown object estimation has been performed using the upper-bound constrained deconvolution. A lateral resolution of ~1-2 micrometer has been demonstrated.
We propose to use high numerical aperture single mode optical fibers like photonic crystal fiber for lensless in-line holographic microscopy. Highly divergent beam helps to overcome the spatial sampling limitation of the image sensor. In this paper, a submicron lateral resolution has been demonstrated, with an imaging sensor of pixel pitch 1.12 micrometer and a photonic crystal fiber of mode field diameter 1.8 micrometer. In earlier methods of single-shot lensless imaging, submicron resolution has been obtained at very small working distance and field of view. The proposed method improves the …
This paper numerically investigates the feasibility of lensless zoomable holographic multiple projections to tilted planes. We have already developed lensless zoomable holographic single projection using scaled diffraction, which calculates diffraction between parallel planes with different sampling pitches. The structure of this zoomable holographic projection is very simple because it does not need a lens; however, it only projects a single image to a plane parallel to the hologram. The lensless zoomable holographic projection in this paper is capable of projecting multiple images onto tilte…
On-chip holographic video is a convenient way to monitor biological samples simultaneously at high spatial resolution and over a wide field-of-view. However, due to the limited readout rate of digital detector arrays, one often faces a tradeoff between the per-frame pixel count and frame rate of the captured video. In this report, we propose a subsampled phase retrieval (SPR) algorithm to overcome the spatial-temporal trade-off in holographic video. Compared to traditional phase retrieval approaches, our SPR algorithm uses over an order of magnitude less pixel measurements while maintaining su…
Traditional microbial detection methods often rely on the overall property of microbial cultures and cannot resolve individual growth event at high spatiotemporal resolution. As a result, they require bacteria to grow to confluence and then interpret the results. Here, we demonstrate the application of an integrated ptychographic sensor for lensless cytometric analysis of microbial cultures over a large scale and with high spatiotemporal resolution. The reported device can be placed within a regular incubator or used as a standalone incubating unit for long-term microbial monitoring. For longi…
University of California, Berkeley（加利福尼亚大学伯克利分校）；UCB/UCSF Joint Graduate Program in Bioengineering（加州大学伯克利分校/加州大学旧金山分校联合生物工程研究生项目）
Hyperspectral imaging is useful for applications ranging from medical diagnostics to agricultural crop monitoring; however, traditional scanning hyperspectral imagers are prohibitively slow and expensive for widespread adoption. Snapshot techniques exist but are often confined to bulky benchtop setups or have low spatio-spectral resolution. In this paper, we propose a novel, compact, and inexpensive computational camera for snapshot hyperspectral imaging. Our system consists of a tiled spectral filter array placed directly on the image sensor and a diffuser placed close to the sensor. Each poi…
Holographic wavefront manipulation enables converting hair-thin multimode optical fibres into minimally invasive lensless imaging instruments conveying much higher information densities than conventional endoscopes. Their most prominent applications focus on accessing delicate environments, including deep brain compartments, and recording micrometre-scale resolution images of structures in close proximity to the distal end of the instrument. Here, we introduce an alternative 'farfield' endoscope, capable of imaging macroscopic objects across a large depth of field. The endoscope shaft with dim…
Spatio-temporally modulated impedance surfaces can be good candidates for generation of radiating waves with arbitrary eigenstates by breaking momentum and energy conservations. Here, we present a theoretical framework based on the holographic technique and generalized Floquet-wave expansion to analyze spatio-temporally modulated impedance surfaces. The holographic technique estimates the required impedance distribution to achieve the desired momentum. Injecting temporal modulation deviates the eigenvalues and changes the radiation frequency. Using the proposed analytical model, the eigenvalue…
Spectral dispersion of ultrashort pulses allows simultaneous focusing of light in both space and time creating so-called spatio-temporal foci. Such space-time coupling may be combined with existing holographic techniques to give a further dimension of control when generating focal light fields. It is shown that a phase-only hologram placed in the pupil plane of an objective and illuminated by a spatially chirped ultrashort pulse can be used to generate three dimensional arrays of spatio-temporally focused spots. Exploiting the pulse front tilt generated at focus when applying simultaneous spat…
We describe an automatic event recognition (AER) system based on a three-dimensional spatio-temporal correlator (STC) that combines the techniques of holographic correlation and photon echo based temporal pattern recognition. The STC is shift invariant in space and time. It can be used to recognize rapidly an event (e.g., a short video clip) that may be present in a large video file, and determine the temporal location of the event. Using polar Mellin transform, it is possible to realize an STC that is also scale and rotation invariant spatially. Numerical simulation results of such a system a…
We present a method of rendering aerial and volumetric graphics using femtosecond lasers. A high-intensity laser excites a physical matter to emit light at an arbitrary 3D position. Popular applications can then be explored especially since plasma induced by a femtosecond laser is safer than that generated by a nanosecond laser. There are two methods of rendering graphics with a femtosecond laser in air: Producing holograms using spatial light modulation technology, and scanning of a laser beam by a galvano mirror. The holograms and workspace of the system proposed here occupy a volume of up t…
The ability to dynamically steer fs pulses from a monolithically integrated source is a critical milestone for the fields of nanophotonics and ultrafast optics. The nascent field of reconfigurable metasurfaces -- made of optically resonant meta-atoms -- has shown great promise in manipulating light-matter interactions through subwavelength control of the phase, amplitude, and polarization of light. These active metasurfaces arbitrarily transform an incident wavefront using a reconfigurable spatial phase profile and thus have been limited to manipulating coherent external sources. Light emittin…
