Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated microscope has made AO prohibitive for its widespread adaptation in microscopy systems. We present here the design and performance of a compact fluorescence microscope using a fully refractive optofluidic wavefront modulator yielding imaging performance on par with that of conventional deformable mirrors, both in correction fidelity and articulation. We combine this device with a modal sensorless wavefront estimation algorithm that uses spatial frequency content of acquired images as a quality metric and thereby demonstrate a completely in-line adaptive optics microscope which can perform aberration correction up to 4$^{th}$ radial order of Zernike modes. This entirely new concept for adaptive optics microscopy may prove to extend the performance limits and widespread applicability of AO in life science imaging.
We present a method to calibrate wavefront distortion of the spatial light modulator setup by registering far field images of several Gaussian beams diffracted off the modulator. The Fourier transform of resulting interference images reveals phase differences between typically 5 movable points on the modulator. Repeating this measurement yields wavefront surface. Next, the amplitude efficiency is calibrated be registering near field image. As a verification we produced a superposition of 7th and 8th Bessel beams with different phase velocities and observed their interference.
We present a liquid crystal method of correcting the phase of an aberrated wavefront using a spatial light modulator. A simple and efficient lab model has been demonstrated for wavefront correction. The crux of a wavefront correcting system in an adaptive optics system lies in the speed and the image quality that can be achieved. The speeds and the accuracy of wavefront representation using Zernike polynomials have been presented using a very fast method of computation.
We propose and demonstrate a method for the adaptive wavefront correction of dynamic multimode fiber beams for the first time. The wavefront of incident beam is reconstructed in real-time based on the complete modal information, which obtained by using the modal decomposition of correlation filter method. For the proof of principle, both of the modal decomposition and the wavefront correction are implemented using the same computer-generated hologram, which encoded into a phase-only spatial light modulator. We demonstrate the wavefront correction of dynamic multimode beam at a rate of 5Hz and …
The controlled modulation of an optical wavefront is required for aberration correction, digital phase conjugation or patterned photostimulation. For most of these applications it is desirable to control the wavefront modulation at the highest rates possible. The digital micromirror device (DMD) presents a cost-effective solution to achieve high-speed modulation and often exceeds the speed of the more conventional liquid crystal spatial light modulator, but is inherently an amplitude modulator. Furthermore, spatial dispersion caused by DMD diffraction complicates its use with pulsed laser sour…
Sorbonne Université, CNRS, Institut des NanoSciences de Paris（索邦大学，法国国家科学研究中心，巴黎纳米科学研究所）；University of the Witwatersrand（威特沃特斯兰德大学）；Università degli Studi di Napoli Federico II（那不勒斯费德里科二世大学）；Scuola Superiore Meridionale（南方高等学院）
We present a minimally-invasive endoscope based on a multimode fiber that combines photoacoustic and fluorescence sensing. From the measurement of a transmission matrix during a prior calibration step, a focused spot is produced and raster-scanned over a sample at the distal tip of the fiber by use of a fast spatial light modulator. An ultra-sensitive fiber-optic ultrasound sensor for photoacoustic detection placed next to the fiber is combined with a photodetector to obtain both fluorescence and photoacoustic images with a distal imaging tip no larger than 250um. The high signal-to-noise rati…
The small correction volume for conventional wavefront shaping methods limits their applications in biological imaging through scattering media. We demonstrate large volume wavefront shaping through a scattering layer with a single correction by conjugate adaptive optics and remote focusing (CAORF). The remote focusing module can keep the conjugation between the AO and scattering layer during three-dimensional scanning. This new configuration provides a wider correction volume by the best utilization of the memory effect in a fast three-dimensional laser scanning microscope. Our results show t…
In this work we compare the Canonical and Hadamard bases for in-situ wavefront correction of a focused Gaussian beam using a spatial light modulator (SLM). The beam is perturbed with a transparent optical element (sparse) or a random scatterer (both prevent focusing at a single spot). The phase corrections are implemented with different basis sizes ($N=64, 256, 1024, 4096$) and the phase contribution of each basis element is measured with 3 step interferometry. The field is reconstructed from the complete $3N$ measurements and the correction is implemented by projecting the conjugate phase at …
Optical focusing through/inside scattering media, like multimode fiber and biological tissues, has significant impact in biomedicine yet considered challenging due to strong scattering nature of light. Previously, promising progress has been made, benefiting from the iterative optical wavefront shaping, with which deep-tissue high-resolution optical focusing becomes possible. Most of iterative algorithms can overcome noise perturbations but fail to effectively adapt beyond the noise, e.g. sudden strong perturbations. Re-optimizations are usually needed for significant decorrelated medium since…
Acquiring high-contrast optical images deep inside biological tissues is still a challenging problem. Confocal microscopy is an important tool for biomedical imaging since it improves image quality by rejecting background signals. However, it suffers from low sensitivity in deep tissues due to light scattering. Recently, multimode fibers have provided a new paradigm for minimally invasive endoscopic imaging by controlling light propagation through them. Here we introduce a combined imaging technique where confocal images are acquired through a multimode fiber. We achieve this by digitally engi…
