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arXiv 1912.02474q-bio.GNq-bio.PE
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装配体ASM291031v2(Genbank:GCA_002910315.2)被鉴定为北方多莉瓦登(Salvelinus malma malma)基因组的组装,而不是北极红点鲑(S.高山植物基因组

Assembly ASM291031v2 (Genbank: GCA_002910315.2) identified as assembly of the Northern Dolly Varden (Salvelinus malma malma) genome, and not the Arctic char (S. alpinus) genome

S. V. Shedko

摘要:

到目前为止,12个完整的基因组,代表11个物种,属于6个属已被测序的鲑鱼。对于红点鲑属,它应该对北极红点鲑的基因组进行测序,北极红点鲑是脊椎动物中变化最大的物种之一。进行测序(Christensen等人,2018)使用从从事工业化养殖木炭的公司- Icy Waters Ltd.获得的雌性IW 2 -2015的组织。该公司利用其自己的两种亲鱼- NL和TR,来自Nauyuk湖和Tree河(Nunavut,加拿大)的木炭。由于雌性IW 2 -2015的完整线粒体基因组在已发表的组装ASM 291031 v2中不存在,我们从Genbank的序列读取档案中确定了其类型和完整序列。结果表明,该雌虫的有丝分裂基因组属于BERING单倍群,这是北方多莉Varden S的特征。马尔玛马尔玛。对核DNA编码的其他非连锁诊断位点(ITS 1、RAG 1、SFO-12、SFO-18、SMM-21)的分析也揭示了雌性IW 2 -2015中北方多莉瓦登的独特特征。由此推断,基因组组装体ASM 291031 v2不是从北极红点鲑个体获得的。alpinus,但来自一个相关物种的个体-北方多莉Varden S。马尔玛马尔玛。在亲鱼TR的其他个体中发现了与IW 2 -2015相同的雌性特征诊断位点。显然,亲鱼TR完全是来自北方多莉瓦登的品种。由于组装体ASM 291031 v2是从来自北多莉瓦登(树R.)分离自该物种的主要分布区,并带有渐渗杂交的痕迹,该组装很难被认为是S.马尔玛马尔玛。

英文摘要:

To date, twelve complete genomes representing eleven species belonging to six genera have been sequenced in salmonids. For the genus Salvelinus, it was supposed to sequence the genome of Arctic char, one of the most variable species of vertebrate animals. Sequencing was carried out (Christensen et al., 2018) using the tissues of the female IW2-2015 obtained from the company engaged in industrial aquaculture of chars - Icy Waters Ltd. The company exploits two of its own broodstocks - NL and TR, originating from the chars from the Nauyuk Lake and the Tree River (Nunavut, Canada). Since the complete mitochondrial genome of the female IW2-2015 was absent in the published assembly ASM291031v2, we determined its type and complete sequence from the sequence read archives taken from Genbank. It was found that the female's mitogenome belongs to the BERING haplogroup, which is characteristic of Northern Dolly Varden S. malma malma. Analysis of other unlinked diagnostic loci encoded by nuclear DNA (ITS1, RAG1, SFO-12, SFO-18, SMM-21) also revealed distinctive characters of Northern Dolly Varden in female IW2-2015. It was concluded that the genomic assembly ASM291031v2 was obtained not from an individual of Arctic char S. alpinus, but from an individual of a related species - Northern Dolly Varden S. malma malma. The identical to the IW2-2015 female characteristics of diagnostic loci were found in other individuals from the broodstock TR. Apparently, the broodstock TR is entirely a strain derived from Northern Dolly Varden. Since assembly ASM291031v2 was obtained from a specimen originated from the marginal population of Northern Dolly Varden (Tree R.) isolated from the main range of the species and with some traces of introgressive hybridization, this assembly can hardly be considered as a description of a typical genome of S. malma malma.

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